Probe:	IMAGE clone 763537
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	8A	Expression is present in most of the neural plate.
TS13: future prosencephalon	Absent		8A	Expression is excluded from the future forebrain.

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	8B	Expression is present in most of the neural plate.
TS13: future prosencephalon	Absent		8B	Expression is excluded from the future forebrain.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	8C	Expression is present in the caudal diencephalon. The rostral limit of expression approximates the zona limitans.
TS15: future midbrain	Present		8C
TS15: future spinal cord	Present		8C
TS15: telencephalon	Present	Regionally restricted	8C	Expression is in the caudodorsal telencephalon.

Structure	Level	Pattern	Image	Note
TS15: telencephalon roof plate	Present		8C'	Expression is present in the dorsal telencephalon.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Widespread	8D	Expression extends throughout the neural tube.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Widespread	8E	Expression extends throughout the neural tube.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Widespread	8F	Expression extends throughout the neural tube.
TS17: telencephalon	Present	Regionally restricted	8F	Expression in the telencephalon is limited to the cerebral cortex; expression in the telencephalic dorsal midline is not detectable.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	8G	Expression in the telencephalon is limited to the cerebral cortex; expression in the telencephalic dorsal midline is not detectable.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	8H	Expression in the telencephalon is limited to the cerebral cortex; expression in the telencephalic dorsal midline is not detectable.
TS17: optic stalk	Present	Regionally restricted	8H	Expression is present in the lateral optic stalk.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		8I
TS17: facial ganglion	Present		8I
TS17: trigeminal ganglion	Present		8I

Structure	Level	Pattern	Image	Note
TS20: brain	Present		8J	Expression is present in the neuroepithelium of most of the neural tube.

Structure	Level	Pattern	Image	Note
TS20: brain	Present		8K	Expression is present in the neuroepithelium of most of the neural tube.
TS20: cerebral cortex	Present	Graded	8K	Expression is present in a dorsal-to-ventral gradient.
TS20: optic stalk	Present	Regionally restricted	8K	Expression is present in the medial optic stalk.

Structure	Level	Pattern	Image	Note
TS20: brain	Present		8L	Expression is present in the neuroepithelium of most of the neural tube.
TS20: epithalamus	Absent		8L	The majority of roofplate derivates show no expression, including the epiphysis.
TS20: choroid plexus	Absent		8L	The majority of roofplate derivates show no expression, including the choroid plexus of the lateral and fourth ventricles.

Structure	Level	Pattern	Image	Note
TS20: brain	Present		8M	Expression is present in the neuroepithelium of most of the neural tube.
TS20: 4th ventricle choroid plexus	Absent		8M	The majority of roofplate derivates show no expression, including the choroid plexus of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS24: brain	Present		9A	Expression is present in the neuroepithelium of most of the neural tube.

Structure	Level	Pattern	Image	Note
TS24: brain	Present		9B	Expression is present in the neuroepithelium of most of the neural tube.
TS24: adenohypophysis	Present		9B	Expression is present in the anterior pituitary.

Structure	Level	Pattern	Image	Note
TS24: brain	Present		9C	Expression is present in the neuroepithelium of most of the neural tube.
TS24: tectum	Strong	Regionally restricted	9C , 9F	Expression is found in the region of the developing subcommissural organ.

Structure	Level	Pattern	Image	Note
TS24: brain	Present		9D	Expression is present in the neuroepithelium of most of the neural tube.

Structure	Level	Pattern	Image	Note
TS22: tectum	Strong	Regionally restricted	9E	Expression is found in the region of the developing subcommissural organ.

Structure	Level	Pattern	Image	Note
TS27: subcommissural organ	Strong		9G	Expression is found in the region of the developing subcommissural organ.