Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:45681
Suda Y, et al., Cooperation between Otx1 and Otx2 genes in developmental patterning of rostral brain. Mech Dev. 1997 Dec;69(1-2):125-41
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5433574
|
|
Gene symbol:
|
Otx2
|
|
Gene name:
|
orthodenticle homeobox 2
|
|
Probe:
|
Otx2 probeA
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
4i:
embryonic day 9.5
(more  )
Specimen
4i:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4j:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Present |
|
4j
|
Expression caudal to the sulcus telodiencephalicus was greatly narrowed. The caudal limit was shifted anteriorly in the double mutant.
|
| TS15: future midbrain |
Present |
|
4j
|
Expression caudal to the sulcus telodiencephalicus was greatly narrowed. The caudal limit was shifted anteriorly in the double mutant.
|
Specimen
6e:
embryonic day 8.0
(more )
Note: 3 somite stage.
Specimen
6e:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
3 somite stage.
|
Specimen
6f:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
3 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural ectoderm |
Present |
|
6f
|
Expression was unchanged in the double mutant at the 3-somite stage.
|
Specimen
6g:
embryonic day 8.0
(more )
Note: 6 somite stage.
Specimen
6g:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
6 somite stage.
|
Specimen
6h:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
6 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural ectoderm |
Present |
Regionally restricted |
6h
|
Expression was greatly limited at the 6-somite stage in the double mutant. The caudal limit of expression was less distinct.
|
|
Analysis Tools