Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45681
Suda Y, et al., Cooperation between Otx1 and Otx2 genes in developmental patterning of rostral brain. Mech Dev. 1997 Dec;69(1-2):125-41
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5433381
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 cDNA13
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3e:
embryonic day 9.5
(more  )
Specimen
3e:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3f:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: midbrain-hindbrain junction |
Present |
Regionally restricted |
3f
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Expression was slightly enlarged and anteriorly shifted. The dorsal expression was enlarged rostrally from the stripe.
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| TS15: diencephalon |
Present |
Regionally restricted |
3f
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Expression was detected in the dorsal diencephalon and commissural plate as in wild type.
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Specimen
8a:
embryonic day 8.0
(more )
Note: 3 somite stage.
Specimen
8a:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3 somite stage.
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Specimen
8b:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3 somite stage.
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Specimen
8c:
embryonic day 8.0
(more )
Note: 6 somite stage.
Specimen
8c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage.
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Specimen
8d:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: midbrain-hindbrain junction |
Present |
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8d
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Expression intensity was increased at 6 somite stage, but not concentrated in the double heterozygotes.
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Analysis Tools