Probe:	Hfh2 cDNA1
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: enteric nervous system	Present	Regionally restricted	3B	Expression was detected in several neural crest cell populations, including cells within the wall of the midgut that are enteric nervous system precursors, future spinal ganglia, and future sympathetic ganglia surrounding the aorta.
TS17: spinal ganglion	Present	Regionally restricted	3B	Expression was detected in several neural crest cell populations, including cells within the wall of the midgut that are enteric nervous system precursors, future spinal ganglia, and future sympathetic ganglia surrounding the aorta.
TS17: sympathetic nervous system	Present	Regionally restricted	3B	Expression was detected in several neural crest cell populations, including cells within the wall of the midgut that are enteric nervous system precursors, future spinal ganglia, and future sympathetic ganglia surrounding the aorta.
TS17: future spinal cord	Present	Regionally restricted	3B , 3C

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Present	Regionally restricted	3D	The expression pattern in the more caudal neural tube differs from that in Fig. 3C. Expression is restricted to the migrating neural crest and the dorsal roof plate.
TS17: neural tube	Present	Regionally restricted	3D	The expression pattern in the more caudal neural tube differs from that in Fig. 3C. Expression is restricted to the migrating neural crest and the dorsal roof plate.
TS17: neural tube roof plate	Present	Regionally restricted	3D	The expression pattern in the more caudal neural tube differs from that in Fig. 3C. Expression is restricted to the migrating neural crest and the dorsal roof plate.

Structure	Level	Pattern	Image	Note
TS17: tail neural tube	Present	Regionally restricted	3E	In the tail region, expression was detected only in cells in the roof plate.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	4A	Expression was detected in a region of the spinal cord thought to give rise to motorneurons.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Diffuse	4B	Expression in the E12.5 spinal cord is more diffuse than at E10.5. There is still a slight concentration of signal remaining in the central region of the spinal cord.