Antibody:	aHox2.1a
Detection system:	Secondary antibody coupled to alkaline phosphatase
Assay notes:	Prior to E8.0 (7 somites) protein was not detected.

Structure	Level	Pattern	Image	Note
TS12: somite 05	Present	Regionally restricted	3B , 3A	Expression is in the posterior half.
TS12: somite 06	Present		3B , 3A
TS12: somite 07	Present		3B , 3A
TS12: neural tube	Present	Regionally restricted	3A , 3B	Expression is anterior.
TS12: future brain	Present	Regionally restricted	3A , 3B	Expression is up to somite 1.

Structure	Level	Pattern	Image	Note
TS13: somite 05	Present	Regionally restricted	3C	Expression is in the posterior half.
TS13: future brain	Present	Regionally restricted	3C	Expression is up to the level of somite 3 and 4.
TS13: neural tube	Present	Regionally restricted	3C	Expression is up to the level of somite 3 and 4.

Structure	Level	Pattern	Image	Note
TS13: somite 05	Present	Regionally restricted	3D	Expression is in the posterior half.
TS13: future brain	Present	Regionally restricted	3D	Expression is up to the level of somite 3 and 4.
TS13: neural tube	Present	Regionally restricted	3D	Expression is up to the level of somite 3 and 4.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present		4A
TS14: neural tube	Present		4A

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Strong	Regionally restricted	4B	Expression is in the lateral plate mesenchyme.
TS15: neural tube	Strong		4B
TS15: head mesenchyme derived from neural crest	Strong		4B , 4D	Expression is in cranial neural crest located posterior and lateral to the otic vesicle at the level of the heart.
TS15: gut	Present		4B
TS15: lung	Present		4B
TS15: forelimb bud	Present	Regionally restricted	4B	Expression is in the anterior proximal region.
TS15: somite	Weak		4B

Structure	Level	Pattern	Image	Note
TS17: vagus ganglion	Present	Regionally restricted	4C	Nodose ganglion placode.
TS17: 3rd branchial arch	Present		4F , 4C
TS17: 4th branchial arch	Present		4F , 4C
TS17: trunk mesenchyme	Weak	Regionally restricted	4C	Expression is in lateral plate mesenchyme.

Structure	Level	Pattern	Image	Note
TS16: lung mesenchyme	Present		4E
TS16: vagus ganglion	Present		4E
TS16: forelimb bud	Present	Regionally restricted	4E	Expression is in the anterior proximal region.
TS16: gut	Present	Regionally restricted	4E	Expression is in mesenchyme.
TS16: somite	Weak		4E

Structure	Level	Pattern	Image	Note
TS20: respiratory tract	Present	Single cells	5A	Cells in the larynx.
TS20: intestine mesenchyme	Present	Regionally restricted	5A	Expression is in the mesoderm of the proximal portion of the small intestine.
TS20: enteric nervous system	Present	Regionally restricted	5B , 5A	The posterior limit is at the point of herniation.
TS20: hindgut	Present		5A
TS20: lung mesenchyme	Present		5A
TS20: stomach mesenchyme	Present		5A
TS20: esophagus mesenchyme	Absent		5A
TS20: trachea mesenchyme	Absent		5A

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	5C	Expression is in the anterior proximal region.

Structure	Level	Pattern	Image	Note
TS16: genitourinary system	Weak	Regionally restricted		Expression is in the metanephric tubules and undifferentiated nephrogenic cord.

Structure	Level	Pattern	Image	Note
TS17: genitourinary system	Present	Regionally restricted		Expression is in metanephric tubules.