Probe:	pBSSK+-Noggin
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: node	Strong		1D1
TS11: notochordal plate	Strong		1D1
TS11: embryo	Present	Regionally restricted	1D1	Expression is absent in the constricting anterior intestinal portal.
TS11: allantois	Absent		1D1
TS11: yolk sac	Absent		1D1

Structure	Level	Pattern	Image	Note
TS12: node	Present		1D2
TS12: notochord	Present		1D2
TS12: notochordal plate	Present		1D2
TS12: future brain neural fold	Present	Regionally restricted	1D2	Expression is in the dorsal tips of the neural folds.
TS12: future spinal cord neural fold	Present	Regionally restricted	1D2	Expression is in the dorsal tips of the neural folds.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	1D3	Expression is dorsal at the fusing and fused neural folds.
TS13: future midbrain	Present	Regionally restricted	1D3	Expression is dorsal at the fusing and fused neural folds.
TS13: future prosencephalon	Present	Regionally restricted	1D3	Expression is dorsal at the fusing and fused neural folds.
TS13: neural tube	Present	Regionally restricted	1D3	Expression is dorsal at the fusing and fused neural folds.
TS13: somite	Present	Regionally restricted	1D3	Expression is in the dorsalmost edge of the somites.
TS13: notochord	Present	Non-Uniform	1D3	Expression declines in the anterior portion of the embryo.
TS13: midbrain-hindbrain junction	Absent		1D3

Structure	Level	Pattern	Image	Note
TS14: optic vesicle	Present	Regionally restricted	1D4	Expression is dorsal.
TS14: somite	Present	Regionally restricted	1D4	Expression is the dorsal rostral portion of the somites.
TS14: future midbrain	Present	Regionally restricted	1D4	Expression is in the most dorsalmost region.
TS14: prosencephalon	Present	Regionally restricted	1D4	Expression is in the most dorsalmost region.
TS14: cardiac muscle tissue	Weak		1D4
TS14: hepatic primordium	Weak		1D4
TS14: midbrain-hindbrain junction	Absent		1D4

Structure	Level	Pattern	Image	Note
TS15: somite	Strong	Regionally restricted	1D5	Expression is in the dorsal tip.
TS15: notochord	Present	Regionally restricted	1D5	Expression is posterior.
TS15: neural tube	Present	Regionally restricted	1D5	Expression is dorsal.
TS15: telencephalon	Present		1D5
TS15: midbrain-hindbrain junction	Absent

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	1D6	Expression is dorsal.
TS17: midbrain-hindbrain junction	Absent		1D6

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	2D1	Expression is dorsal at the fusing and fused neural folds.
TS13: future midbrain	Present	Regionally restricted	2D1	Expression is dorsal at the fusing and fused neural folds.
TS13: future prosencephalon	Present	Regionally restricted	2D1	Expression is dorsal at the fusing and fused neural folds.
TS13: neural tube	Present	Regionally restricted	2D1	Expression is dorsal at the fusing and fused neural folds.
TS13: midbrain-hindbrain junction	Absent		2D1

Structure	Level	Pattern	Image	Note
TS14: optic vesicle	Present	Regionally restricted	2D2	Expression is dorsal.
TS14: future midbrain	Present	Regionally restricted	2D2	Expression is in the most dorsalmost region.
TS14: prosencephalon	Present	Regionally restricted	2D2	Expression is in the most dorsalmost region.
TS14: cardiac muscle tissue	Weak		2D2

Structure	Level	Pattern	Image	Note
TS14: somite	Present		2D3	Expression is the dorsal rostral portion of the somites.

Structure	Level	Pattern	Image	Note
TS17: gut	Absent		2D4

Structure	Level	Pattern	Image	Note
TS17: heart	Absent		2D5

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2D6	Expression is dorsal.
TS17: somite	Weak		2D6

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	3D1	Expression is dorsal at the fusing and fused neural folds.
TS13: future midbrain	Present	Regionally restricted	3D1	Expression is dorsal at the fusing and fused neural folds.
TS13: future prosencephalon	Present	Regionally restricted	3D1	Expression is dorsal at the fusing and fused neural folds.
TS13: neural tube	Present	Regionally restricted	3D1	Expression is dorsal at the fusing and fused neural folds.

Structure	Level	Pattern	Image	Note
TS14: cardiac muscle tissue	Weak		3D2

Structure	Level	Pattern	Image	Note
TS14: limb	Absent		3D3

Structure	Level	Pattern	Image	Note
TS17: stomach	Absent		3D4

Structure	Level	Pattern	Image	Note
TS17: lung	Present		3D5

Structure	Level	Pattern	Image	Note
TS13: future brain	Strong	Regionally restricted		Expression is in the seam.
TS13: neural fold	Moderate
TS13: future hindbrain	Weak
TS13: future midbrain	Weak
TS13: heart	Weak
TS13: somite	Weak
TS13: future prosencephalon	Present	Regionally restricted		Expression is at the fusing and fused neural folds and is absent in the telencephalon.

Structure	Level	Pattern	Image	Note
TS14: future brain	Strong	Regionally restricted		Expression is in the seam.
TS14: future hindbrain	Present
TS14: future midbrain	Present
TS14: somite	Present
TS14: prosencephalon	Present	Regionally restricted		Expression is in telencephalon.
TS14: heart	Weak
TS14: limb	Absent

Structure	Level	Pattern	Image	Note
TS17: brain	Strong	Regionally restricted		Expression is in seam.
TS17: hindbrain	Moderate
TS17: midbrain	Moderate
TS17: telencephalon	Moderate
TS17: lung	Weak
TS17: somite	Weak
TS17: heart	Absent
TS17: limb	Absent
TS17: stomach	Absent