Probe:	pCMV5Sport6-Bmp4
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS10: allantois	Strong		1B1
TS10: yolk sac	Strong		1B1
TS10: embryo	Strong	Regionally restricted	1B1	Expression is in the anterior part including the cardiac crescent.
TS10: neural ectoderm	Strong	Regionally restricted	1B1	Expression is in the early neural folds.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Strong	Regionally restricted	1B2	Expression is posterior.
TS12: allantois	Present		1B2
TS12: yolk sac	Present		1B2
TS12: foregut diverticulum	Strong	Regionally restricted	1B2	Expression is in the constricting anterior intestinal portal.
TS12: sinus venosus	Strong		1B2

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Strong	Regionally restricted	1B3	Expression is posterior.
TS13: allantois	Strong		1B3
TS13: sinus venosus	Strong		1B3
TS13: yolk sac	Strong		1B3
TS13: embryo	Present	Regionally restricted	1B3	Expression is in the caudal most mesoderm of the tail.
TS13: future prosencephalon	Present	Regionally restricted	1B3	Expression is in the seam of the diencephalon.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	1B4	Expression is at the midline seam of the telencephalon.
TS14: branchial groove	Present		1B4
TS14: future adenohypophysis	Present		1B4
TS14: hepatic primordium	Present		1B4
TS14: limb	Present		1B4
TS14: otic pit	Present		1B4
TS14: thyroid primordium	Present		1B4
TS14: somite	Present	Regionally restricted	1B4	Rostral somites.
TS14: branchial arch	Present	Regionally restricted	1B4	Expression is at the tip.
TS14: heart	Present	Regionally restricted	1B4	Expression is at the sinoatrial region.
TS14: optic vesicle	Present	Regionally restricted	1B4	Expression is dorsal.
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted	1B4	Expression is posterior.
TS14: 1st branchial arch	Present	Regionally restricted	1B4	Expression is in the distal portion.

Structure	Level	Pattern	Image	Note
TS15: somite	Strong	Regionally restricted	1B5	Expression is in the rostral dorsal somites.
TS15: telencephalon	Strong	Regionally restricted	1B5	Expression is in the seam of the telencephalon.
TS15: branchial arch	Strong		1B5
TS15: branchial arch mesenchyme derived from neural crest	Strong		1B5
TS15: optic cup	Strong	Regionally restricted	1B5	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Strong		1B6
TS17: apical ectodermal ridge	Strong		1B6
TS17: lung	Strong		1B6
TS17: outflow tract	Strong		1B6
TS17: telencephalon	Strong		1B6
TS17: nose	Strong	Regionally restricted	1B6	Expression is around the nasal placode.
TS17: somite	Strong	Regionally restricted	1B6	Expression is in the dorsal somite spannning the entire length of the embryo.
TS17: gut	Strong	Regionally restricted	1B6	Expression is in dorsal gut tube mesoderm.
TS17: limb	Strong	Regionally restricted	1B6	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present	Regionally restricted	2B1	Expression is in the dorsalmost tips of the rostral neural tube.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	2B2	Expression is at the midline seam of the telencephalon.
TS14: branchial groove	Present		2B2
TS14: future adenohypophysis	Present		2B2
TS14: otic pit	Present		2B2
TS14: thyroid primordium	Present		2B2
TS14: optic vesicle	Present	Regionally restricted	2B2	Expression is dorsal.
TS14: heart	Present	Regionally restricted	2B2	Expression is at the sinoatrial region.
TS14: branchial arch	Present	Regionally restricted	2B2	Expression is at the tip.
TS14: 1st branchial arch	Present	Regionally restricted	2B2	Expression is in the distal portion.

Structure	Level	Pattern	Image	Note
TS14: limb	Present		2B3

Structure	Level	Pattern	Image	Note
TS17: gut	Strong		2B4	Expression is in dorsal gut tube mesoderm.

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Strong		2B5

Structure	Level	Pattern	Image	Note
TS17: somite	Strong	Regionally restricted	2B6	Expression is in the dorsal somite spannning the entire length of the embryo.

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present	Regionally restricted	3B1	Expression is in the dorsalmost tips of the rostral neural tube.

Structure	Level	Pattern	Image	Note
TS14: outflow tract	Present		3B2
TS14: sinus venosus	Present		3B2

Structure	Level	Pattern	Image	Note
TS14: limb	Present	Regionally restricted	3B3	Expression is dorsal.
TS14: forelimb bud ectoderm	Present	Regionally restricted	3B3	Expression is in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS17: midgut	Present		3B4

Structure	Level	Pattern	Image	Note
TS17: lung	Present		3B5

Structure	Level	Pattern	Image	Note
TS13: somite	Present
TS13: future midbrain	Weak
TS13: heart	Weak
TS13: neural fold	Weak
TS13: future brain	Weak	Regionally restricted		Expression is in the seam.
TS13: future prosencephalon	Weak	Regionally restricted		Expression is in telencephalon.
TS13: future hindbrain	Absent

Structure	Level	Pattern	Image	Note
TS14: limb	Strong
TS14: future brain	Moderate	Regionally restricted		Expression is in the seam.
TS14: prosencephalon	Present	Regionally restricted		Expression is in telencephalon.
TS14: future midbrain	Weak
TS14: heart	Weak
TS14: somite	Weak
TS14: future hindbrain	Absent

Structure	Level	Pattern	Image	Note
TS17: limb	Strong
TS17: lung	Strong
TS17: somite	Strong
TS17: stomach	Strong
TS17: heart	Moderate
TS17: telencephalon	Moderate
TS17: hindbrain	Present
TS17: brain	Present	Regionally restricted		Expression is in the seam.
TS17: midbrain	Weak