Gene Expression Data
Assay Details
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Assay
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Reference:
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J:123544
Hume CR, et al., Expression of LHX3 and SOX2 during mouse inner ear development. Gene Expr Patterns. 2007 Aug;7(7):798-807
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5431543
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Gene symbol:
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Sox2
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Gene name:
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SRY (sex determining region Y)-box 2
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Results
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Specimen
2Q:
embryonic day 17.0
(more  )
Note: Double labeled: red - Sox2; green - Myo6 (assay MGI:5431532).
Specimen
2Q:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sox2; green - Myo6 (assay MGI:5431532).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cochlea |
Present |
Regionally restricted |
2Q
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Along the medial to lateral axis of the mid-basal cochlea, expressing nuclei are detected in both hair cell and support cell layers spanning from inner hair cells and inner phalangeal cells to outer hair cells, Deiters' cells and lateral tunnel cells.
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Specimen
2R:
embryonic day 17.0
(more )
Note: Double labeled: red - Sox2; green - Prox1 (assay MGI:5431544).
Specimen
2R:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sox2; green - Prox1 (assay MGI:5431544).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cochlea |
Present |
Regionally restricted |
2R
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Along the medial to lateral axis of the mid-basal cochlea, expressing nuclei are detected in both hair cell and support cell layers spanning from inner hair cells and inner phalangeal cells to outer hair cells, Deiters' cells and lateral tunnel cells.
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Specimen
4A:
postnatal adult
(more )
Note: Double labeled: red - Sox2; green - Myo7a (assay MGI:5431556).
Specimen
4A:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sox2; green - Myo7a (assay MGI:5431556).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: utricle epithelium |
Present |
Regionally restricted |
4A
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Expression was detected in a subset of hair cell nuclei distributed across the sensory epithelia.
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Specimen
4D:
postnatal adult
(more )
Note: Double labeled: red - Sox2; green - Myo7a (assay MGI:5431556).
Specimen
4D:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sox2; green - Myo7a (assay MGI:5431556).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: crista ampullaris |
Present |
Regionally restricted |
4D
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Expression was detected in a subset of hair cell nuclei distributed across the sensory epithelia.
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Specimen
4G:
postnatal adult
(more )
Note: Double labeled: red - Sox2; green - Myo7a (assay MGI:5431556).
Specimen
4G:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sox2; green - Myo7a (assay MGI:5431556).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
Present |
Regionally restricted |
4G
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Expression was detected in the auditory sensory epithelium.
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Specimen
5A:
postnatal adult
(more )
Note: Double labeled: red - Sox2; green - Calb1 (assay MGI:5431728).
Specimen
5A:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sox2; green - Calb1 (assay MGI:5431728).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: macula of saccule |
Present |
Regionally restricted |
5A
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Expression was limited to Type II vestibular hair cells in the striola. In extrastriolar regions, approximately one-half of the hair cell nuclei were labeled.
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Specimen
5B:
postnatal adult
(more )
Note: Double labeled: red - Sox2; green - Calb1 (assay MGI:5431728).
Specimen
5B:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sox2; green - Calb1 (assay MGI:5431728).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: saccule |
Present |
Uniform |
5B
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Expression was uniform in support cells.
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Specimen
5C:
postnatal adult
(more )
Note: Double labeled: red - Sox2; green - Pvalb (assay MGI:5431729).
Specimen
5C:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sox2; green - Pvalb (assay MGI:5431729).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
5C
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Expression was detected in a subset of differentiated support cells, including Deiters', pillar, inner phalangeal, tectal and lateral tunnel cells. Expression was absent from adult cochlear hair cells.
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Specimen
5D left:
postnatal adult
(more )
Specimen
5D left:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
5D left
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Expression was detected in a subset of differentiated support cells, including Deiters', pillar, inner phalangeal, tectal and lateral tunnel cells. Expression was absent from adult cochlear hair cells.
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