Antibody:	Anti-Sox2 (Y-17; sc-17320)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS20: saccule epithelium	Present

Structure	Level	Pattern	Image	Note
TS21: saccule epithelium	Present	Regionally restricted	1A	Expressing nuclei span the full thickness of the sensory epithelium.

Structure	Level	Pattern	Image	Note
TS22: saccule epithelium	Present		1E

Structure	Level	Pattern	Image	Note
TS23: saccule epithelium	Present		1I

Structure	Level	Pattern	Image	Note
TS23: greater epithelial ridge	Present	Regionally restricted	1M	Expression was limited to a domain that encompasses parts of adjacent areas of both the lesser epithelial ridge and greater epithelial ridge. Immunoreactivity was more intense in the lesser epithelial ridge.
TS23: lesser epithelial ridge	Present	Regionally restricted	1M	Expression was limited to a domain that encompasses parts of adjacent areas of both the lesser epithelial ridge and greater epithelial ridge. Immunoreactivity was more intense in the lesser epithelial ridge.

Structure	Level	Pattern	Image	Note
TS25: saccule epithelium	Present	Regionally restricted	2A	Expression was detected in both hair cell and support cells nuclei in the vestibular epithelia.

Structure	Level	Pattern	Image	Note
TS25: cochlea epithelium	Present	Regionally restricted	2E	Expression was detected in a longitudinal axial band of nuclei spanning the full depth of the epithelium above the basement memrane from the base to apex.

Structure	Level	Pattern	Image	Note
TS25: cochlea epithelium	Present	Regionally restricted	2I	Expression was detected in a longitudinal axial band of nuclei spanning the full depth of the epithelium above the basement memrane from the base to apex.

Structure	Level	Pattern	Image	Note
TS25: cochlea epithelium	Present	Regionally restricted	2M	Expression was detected in a longitudinal axial band of nuclei spanning the full depth of the epithelium above the basement memrane from the base to apex.

Structure	Level	Pattern	Image	Note
TS27: saccule epithelium	Present	Regionally restricted	3A	Expression was declining in the majority of vestibular hair cells, but was maintained in support cells and a subset of hair cells.

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present	Regionally restricted	3E	Expression was expanded medially into the greater epithelial ridge and maintained in support cells (inner phalangeal cells, pillar cells, Deiters' cells, Hensen's cells). Expression is lost from the nuclei of hair cells, but persists in cytoplasm.

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present		3I

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present		3M

Structure	Level	Pattern	Image	Note
TS26: inner ear	Present		S1_SOX2 (left)