Antibody:	clone 39.4D5
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Two antibodies were described, but the paper did not detail which was used in this assay. MGI:1314839 may have been used.

Structure	Level	Pattern	Image	Note
TS28: stomach squamous epithelium	Absent		1B

Structure	Level	Pattern	Image	Note
TS28: stomach glandular region epithelium	Present	Scattered	1C	Positive cells were more numerous in the corpus than the antrum region.

Structure	Level	Pattern	Image	Note
TS28: duodenum epithelium	Present	Scattered	1D

Structure	Level	Pattern	Image	Note
TS28: jejunum epithelium	Present	Scattered	1E

Structure	Level	Pattern	Image	Note
TS28: ileum epithelium	Present	Scattered	1F

Structure	Level	Pattern	Image	Note
TS28: colon epithelium	Present	Scattered	1G

Structure	Level	Pattern	Image	Note
TS28: stomach	Present	Regionally restricted	2C , 2A	Not all Chromogranin A+ cells were positive for Isl1.

Structure	Level	Pattern	Image	Note
TS28: stomach	Present	Regionally restricted	2D , 2F	Most Somatostatin+ cells were positive for Isl1.

Structure	Level	Pattern	Image	Note
TS28: stomach	Present		2G	Most serotonin+ cells were positive for Isl1.

Structure	Level	Pattern	Image	Note
TS28: stomach	Present	Regionally restricted	2J , 2L	Most Ghrelin+ cells were positive for Isl1.

Structure	Level	Pattern	Image	Note
TS28: stomach	Present	Regionally restricted	2M , 2O	Some Gastrin+ cells were also positive for Isl1.

Structure	Level	Pattern	Image	Note
TS28: stomach	Present	Regionally restricted	2P	Immunopositive cells that co-expressed Somatostatin and Ghrelin were detected.

Structure	Level	Pattern	Image	Note
TS28: duodenum epithelium	Present	Single cells	3A , 3C	Very few immunopositive cells were detected but most of them were also positive for Ghrelin expression.

Structure	Level	Pattern	Image	Note
TS28: duodenum epithelium	Present	Single cells	3F , 3D	Very few immunopositive cells were detected but most of them were also positive for Somatostatin expression.

Structure	Level	Pattern	Image	Note
TS28: jejunum epithelium	Present	Single cells	3G , 3I	Very few immunopositive cells were detected but most of them were also positive for Somatostatin expression.

Structure	Level	Pattern	Image	Note
TS28: ileum epithelium	Present	Single cells	3J , 3L	Very few immunopositive cells were detected but most of them were also positive for Somatostatin expression.

Structure	Level	Pattern	Image	Note
TS28: colon epithelium	Present	Single cells	3M , 3O	Very few immunopositive cells were detected but most of them were also positive for Somatostatin expression.

Structure	Level	Pattern	Image	Note
TS15: foregut mesenchyme	Present	Regionally restricted		Expression was first observed in the ventral gastric mesenchyme at E9.5.

Structure	Level	Pattern	Image	Note
TS17: stomach mesenchyme	Present	Regionally restricted	4B	Expression was detected in the ventral and lateral mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: stomach mesenchyme	Present	Uniform	4C	Expression was distributed uniformly in the mesenchyme surrounding the entire gastric epithelium.

Structure	Level	Pattern	Image	Note
TS20: stomach mesenchyme	Present	Regionally restricted	4D	Greater expression was detected in the dorsal mesenchyme than in the ventral mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: stomach mesenchyme	Present		4E	Expression level was higher in the mesenchyme than in the epithelium.
TS21: stomach epithelium	Present		4E	Expression in the gastric epithelium is first detected at E13.5.

Structure	Level	Pattern	Image	Note
TS22: stomach	Present	Regionally restricted	4F' , 4F	Expression was expanded to the entire posterior region of the stomach at E14.5.

Structure	Level	Pattern	Image	Note
TS24: stomach epithelium	Present	Scattered	4G	After epithelial invaginations formed in the gastric epithelium, a few scattered immunopositive cells were found in the base and middle of each gastric unit.

Structure	Level	Pattern	Image	Note
TS25: stomach epithelium	Present	Scattered	4H	After epithelial invaginations formed in the gastric epithelium, a few scattered immunopositive cells were found in the base and middle of each gastric unit.

Structure	Level	Pattern	Image	Note
TS28: stomach epithelium	Present	Scattered	4I	After epithelial invaginations formed in the gastric epithelium, a few scattered immunopositive cells were found in the base and middle of each gastric unit.

Structure	Level	Pattern	Image	Note
TS23: intestine epithelium	Present	Regionally restricted	4J	Expression was first detected in intestinal epithelium at E15.5.

Structure	Level	Pattern	Image	Note
TS25: intestine epithelium	Present	Scattered	4K	Higher expression level was detected in the proximal region of the intestine compared with the distal region.

Structure	Level	Pattern	Image	Note
TS25: intestine epithelium	Present	Scattered	4L	Higher expression level was detected in the proximal region of the intestine compared with the distal region.

Structure	Level	Pattern	Image	Note
TS25: stomach	Present		5A , 5C	Only a few Isl1/chromogranin A double positive cells were noted.

Structure	Level	Pattern	Image	Note
TS28: stomach	Present		5D	Some Isl1+/somatostatin+/ghrelin+ cells were detected.

Structure	Level	Pattern	Image	Note
TS25: stomach epithelium	Present		6D , 6F	Expression did not overlap with Pdx2 in the gastric epithelium at E17.5.

Structure	Level	Pattern	Image	Note
TS28: stomach	Present		6G , 6I	Expression did not overlap with Pdx2 in the adult stomach.

Structure	Level	Pattern	Image	Note
TS28: duodenum	Present		6L , 6J	Expression did not overlap with Pdx2 in the adult intestine.

Structure	Level	Pattern	Image	Note
TS21: stomach epithelium	Present		7A , 7C	Expression did not overlap with Ngn3.

Structure	Level	Pattern	Image	Note
TS23: intestine epithelium	Present		7F , 7D	Expression did not overlap with Ngn3 in the proximal intestine.

Structure	Level	Pattern	Image	Note
TS28: duodenum epithelium	Present		7G , 7I	Expression did not overlap with Ngn3.

Structure	Level	Pattern	Image	Note
TS25: stomach epithelium	Present		7L , 7J	Expression did not overlap with Pax6 in the gastric epithelium.

Structure	Level	Pattern	Image	Note
TS28: stomach epithelium	Present			Expression did not overlap with Pax6 in the gastric epithelium.

Structure	Level	Pattern	Image	Note
TS28: stomach epithelium	Present		7M , 7O	Some Isl1+/Pax6+ co-expressing cells were detected in the adult.

Structure	Level	Pattern	Image	Note
TS22: stomach epithelium	Present		8A	Expression did not overlap with Ki67.

Structure	Level	Pattern	Image	Note
TS25: intestine epithelium	Present		8B	Expression did not overlap with Ki67 in the proximal intestine.

Structure	Level	Pattern	Image	Note
TS25: intestine epithelium	Present		8C	Expression did not overlap with Ki67 in the distal intestine.

Structure	Level	Pattern	Image	Note
TS28: stomach epithelium	Present		8D	Expression did not overlap with Ki67.

Structure	Level	Pattern	Image	Note
TS28: duodenum epithelium	Present		8E	Expression did not overlap with Ki67.

Structure	Level	Pattern	Image	Note
TS28: ileum epithelium	Present		8F	Expression did not overlap with Ki67.

Structure	Level	Pattern	Image	Note
TS28: stomach epithelium	Present		8G	Expression did not overlap with Ki67.

Structure	Level	Pattern	Image	Note
TS28: duodenum epithelium	Present		8H	Expression did not overlap with Ki67.

Structure	Level	Pattern	Image	Note
TS28: ileum epithelium	Present		8I	Expression did not overlap with Ki67.