Probe:	Mrf4 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS14: myotome	Present	Regionally restricted	1A	Expression is in the central region of the myotome of rostral somites. The 10-12 most caudal somites are negative.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	1B , 1C	Expression is in the dorsal half of the 14-15 rostral-most somites with only 6-8 negative somites.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	1D	Epaxial expression and a separate ventral expression domain appears in the hypaxial region of rostral thoracic somites.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	1E	Expression in the ventral expression domain encroaches on the more recently born somites and is already on in the second recently formed somite.
TS15: dermomyotome	Present		1E

Structure	Level	Pattern	Image	Note
TS15: myotome	Present	Regionally restricted	1F	Absent in lumbo-sacral somites, expression in the older thoracic somites across the full dorso-ventral extent of the myotome and cells in the hypaxial domain are becoming spindle shaped.
TS15: somite	Present	Regionally restricted	1F	Absent in lumbo-sacral somites, expression in the older thoracic somites across the full dorso-ventral extent of the myotome and cells in the hypaxial domain are becoming spindle shaped.

Structure	Level	Pattern	Image	Note
TS16: tail somite	Present	Regionally restricted	1G	Epaxial expression preceeds hypaxial expression in lumbo-sacral and tail somites.
TS16: trunk somite	Present	Regionally restricted	1G	Epaxial expression preceeds hypaxial expression in lumbo-sacral and tail somites.

Structure	Level	Pattern	Image	Note
TS15: somite	Present		2_ss25

Structure	Level	Pattern	Image	Note
TS15: trunk myotome	Present	Regionally restricted	2_So XVIII	Expression is in the epaxial myotome.

Structure	Level	Pattern	Image	Note
TS15: trunk myotome	Present	Regionally restricted	2_So XIV	Expression is in the epaxial myotome.

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present	Regionally restricted	2_So XIII	The epaxial expression domain expands ventrally and dorsally towards the dermomyotomal lip.
TS15: trunk myotome	Present	Regionally restricted	2_So XIII	The epaxial expression domain expands ventrally and dorsally towards the dermomyotomal lip.

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present	Regionally restricted	2_So VIII	Expression is in the hypaxial dermomyotome extending into the myotome.
TS15: trunk myotome	Present	Regionally restricted	2_So VIII	Expression is in the hypaxial dermomyotome extending into the myotome.

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present	Regionally restricted	2_So V	Expression is in the hypaxial dermomyotome.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	2_So II	There is little or no expression in the second most recently-formed somite.

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present	Regionally restricted	3_ss27	Expression is in hypaxial dermomyotome of the second most recently formed somite and in older somites.
TS15: somite	Present		3_ss27

Structure	Level	Pattern	Image	Note
TS15: trunk myotome	Present		3_So XI	In this mature somite expression is throughout the epaxial and hypaxial myotome with limited expression in the somitic bud.

Structure	Level	Pattern	Image	Note
TS15: somite	Present		3_So III