Probe:	Ngn2 probe10
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2B	Expression was detected in regions of the dorsal-ventral axis of neuroepithelium overlapping with Nedd9 expression. Expression was seen in the emerging marginal zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2E	Expression is found in 2 discrete dorso-ventral domains. There is some overlap with Nedd9 expression in the ventricular zone. Highest expression was in the non-overlapping intermediate zone.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	2G	Expression was uniformly distributed in the dorso-ventral axis. Most labeled cells were detected in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		3F

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		3G

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	4G	Expression was detected in the hypoglossal nucleus.