Gene Expression Data
Assay Details
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Assay
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Reference:
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J:183735
Moe K, et al., Developmentally regulated expression of Sema3A chemorepellant in the developing mouse incisor. Acta Odontol Scand. 2012 May;70(3):184-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5427633
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Gene symbol:
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Sema3a
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Gene name:
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sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
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Probe:
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Sema3a cDNA3
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1A 1/2:
embryonic day 11.5
(more  )
Specimen
1A 1/2:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: oral epithelium |
Present |
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1A 1/2
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| TS19: mandibular process mesenchyme |
Present |
Regionally restricted |
1A 1/2
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Expressed in the oral mesenchyme including the presumptive area of the incisor tooth germs. No expression in the middle part of the mandibular process mesenchyme.
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Specimen
1B 1/2:
embryonic day 12.5
(more )
Specimen
1B 1/2:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: oral epithelium |
Present |
Regionally restricted |
1B 1/2
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Expressed in the oral epithelium. Expression was mostly absent from the invaginated early epithelial dental bud.
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| TS20: jaw primordium mesenchyme |
Present |
Regionally restricted |
1B 1/2
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Expressed in the presumptive incisor mesenchyme.
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| TS20: Meckel's cartilage |
Strong |
Regionally restricted |
1B 1/2
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Expressed in the mesenchymal condensates of the Meckel's cartilage.
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Specimen
1C 1/2:
embryonic day 13.5
(more )
Specimen
1C 1/2:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: incisor epithelium |
Strong |
Regionally restricted |
1C 1/2
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Expressed in the central part of the epithelial bud.
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| TS21: alveolar sulcus |
Strong |
Regionally restricted |
1C 1/2
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Expressed in the alveolar mesenchyme surrounding the tooth germ.
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| TS21: incisor mesenchyme |
Present |
Regionally restricted |
1C 1/2
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Expressed in the condensed dental mesenchyme (future dental pulp). No expression in the presumptive dental follicle target area (future periodontal ligament).
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Specimen
1D 1/2:
embryonic day 14.5
(more )
Specimen
1D 1/2:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1E 1/2:
embryonic day 16.5
(more )
Specimen
1E 1/2:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2A 1/2:
postnatal day 0
(more )
Specimen
2A 1/2:
(close )
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Genetic Background:
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NMRI
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Age:
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postnatal day 0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2B 1/2:
postnatal day 4
(more )
Specimen
2B 1/2:
(close )
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Genetic Background:
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NMRI
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Age:
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postnatal day 4
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: outer enamel epithelium |
Present |
Regionally restricted |
2B 1/2
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Expressed in outer dental epithelia both on the labial and lingual side of the tooth germ.
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| TS28: alveolar process |
Strong |
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2B 1/2
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Expressed in the alveolar bone around the tooth germ.
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| TS28: dental pulp |
Absent |
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2B 1/2
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No expression in the mesenchymal cells of the dental pulp including the area of the primary apical foramen.
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| TS28: dental sac |
Absent |
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2B 1/2
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No expression in the dental follicle target area.
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| TS28: cervical loop |
Present |
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2B 1/2
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Specimen
2C 1/2:
postnatal day 5
(more )
Specimen
2C 1/2:
(close )
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Genetic Background:
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NMRI
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Age:
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postnatal day 5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: outer enamel epithelium |
Present |
Regionally restricted |
2C 1/2
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Expressed in outer dental epithelia both on the labial and lingual side of the tooth germ.
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| TS28: alveolar process |
Strong |
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2C 1/2
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Expressed in the alveolar bone around the tooth germ.
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| TS28: dental pulp |
Absent |
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2C 1/2
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No expression in the mesenchymal cells of the dental pulp including the area of the primary apical foramen.
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| TS28: dental sac |
Absent |
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2C 1/2
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No expression in the dental follicle target area.
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| TS28: cervical loop |
Present |
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2C 1/2
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Analysis Tools