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Gene Expression Data
Assay Details
Assay
Reference: J:122989 Visel A, et al., GenePaint.org: an atlas of gene expression patterns in the mouse embryo. Nucleic Acids Res. 2004 Jan 1;32(Database issue):D552-6
Assay type: RNA in situ
MGI Accession ID: MGI:5425139
Gene symbol: Hsd3b8
Gene name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 8
Probe: EH716 probe 655
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramide-biotin/streptavidin amplification step was included in the in situ hybridization procedure. These data were downloaded from GenePaint.org on 1/30/12. The probe used in this assay may recognize multiple related genes; see assays MGI:5425138 (Gm4450) and MGI:5423024 (Hsd3b4).
Results
Specimen EH716; Specimen C2401: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: nervous system Strong Regionally restricted Embryo_C2401_4_5A
TS22: central nervous system Strong Regionally restricted Embryo_C2401_4_5A
TS22: telencephalon Strong Regionally restricted Embryo_C2401_4_5A
TS22: cerebral cortex Strong Regionally restricted Embryo_C2401_4_5A
TS22: neocortex Strong Regionally restricted Embryo_C2401_4_5A
TS22: olfactory cortex Strong Regionally restricted Embryo_C2401_4_5A
TS22: main olfactory bulb Strong Regionally restricted Embryo_C2401_4_5A
TS22: piriform cortex Moderate Scattered Embryo_C2401_4_1D
TS22: hippocampus Strong Regionally restricted Embryo_C2401_4_2C
TS22: basal ganglia Strong Regionally restricted Embryo_C2401_4_5A
TS22: corpus striatum Strong Regionally restricted Embryo_C2401_4_2C Expression was strong in the corpus striatum (lateral ganglionic eminence).
TS22: basal forebrain Absent
TS22: amygdala Strong Regionally restricted Embryo_C2401_4_1D
TS22: telencephalon septum Strong Regionally restricted Embryo_C2401_4_5A
TS22: pallidum Strong Regionally restricted Embryo_C2401_4_5A Expression was strong in the pallidum (medial ganglionic eminence).
TS22: diencephalon Absent
TS22: thalamus Absent
TS22: hypothalamus Absent
TS22: midbrain Strong Regionally restricted Embryo_C2401_4_5A
TS22: pretectum Absent
TS22: superior colliculus Moderate Regionally restricted Embryo_C2401_4_4B
TS22: inferior colliculus Moderate Regionally restricted Embryo_C2401_4_4B
TS22: midbrain tegmentum Strong Regionally restricted Embryo_C2401_4_5A
TS22: hindbrain Strong Regionally restricted Embryo_C2401_4_5A
TS22: cerebellum Strong Regionally restricted Embryo_C2401_4_5A
TS22: pons Strong Regionally restricted Embryo_C2401_4_5A
TS22: medulla oblongata Absent
TS22: spinal cord Absent
TS22: choroid plexus Absent
TS22: meninges Absent
TS22: peripheral nervous system Strong Regionally restricted Embryo_C2401_4_5A
TS22: cranial ganglion Moderate Scattered Embryo_C2401_4_2B
TS22: dorsal root ganglion Strong Regionally restricted Embryo_C2401_4_5A
TS22: eye Strong Regionally restricted Embryo_C2401_4_1A
TS22: retina Absent
TS22: lens Strong Regionally restricted Embryo_C2401_4_1A
TS22: cornea Absent
TS22: optic nerve Absent
TS22: nose Absent
TS22: olfactory epithelium Absent
TS22: ear Absent
TS22: inner ear Absent
TS22: cochlea Absent
TS22: inner ear labyrinth Absent
TS22: middle ear Absent
TS22: alimentary system Moderate Regionally restricted Embryo_C2401_4_1B Expression was detected in the digestive system.
TS22: intestine Absent
TS22: salivary gland Absent
TS22: tongue Absent
TS22: tooth Weak Regionally restricted Embryo_C2401_4_4C
TS22: palatal shelf Absent
TS22: esophagus Weak Regionally restricted Embryo_C2401_4_4A
TS22: stomach Weak Regionally restricted Embryo_C2401_4_5B
TS22: liver Moderate Scattered Embryo_C2401_4_1B
TS22: respiratory system Absent
TS22: pharynx Absent
TS22: larynx Absent
TS22: trachea Absent
TS22: main bronchus Absent
TS22: lung Absent
TS22: urinary system Moderate Regionally restricted Embryo_C2401_4_4D Expression was detected in the genito-urinary system.
TS22: metanephros Absent
TS22: ureter Moderate Regionally restricted Embryo_C2401_4_4D
TS22: bladder Absent
TS22: urethra Absent
TS22: reproductive system Absent
TS22: genital tubercle Absent
TS22: gonad Absent
TS22: gland Absent Expression was not detected in the endocrine system.
TS22: thyroid gland Absent
TS22: pituitary gland Absent
TS22: pancreas Absent
TS22: adrenal gland Absent
TS22: cardiovascular system Moderate Regionally restricted Embryo_C2401_4_4A
TS22: heart Moderate Regionally restricted Embryo_C2401_4_4A
TS22: heart atrium Moderate Regionally restricted Embryo_C2401_4_4A
TS22: heart ventricle Moderate Regionally restricted Embryo_C2401_4_4A
TS22: blood vessel Absent
TS22: hemolymphoid system Weak Regionally restricted Embryo_C2401_4_3C Expression was weak in the immune system.
TS22: spleen primordium Absent
TS22: thymus primordium Weak Scattered Embryo_C2401_4_3C
TS22: appendicular skeleton Absent Expression was not detected in the skeleton (limbs, shoulder, hip).
TS22: musculature Absent
TS22: face Absent Expression was not detected in the face/soft tissues.
TS22: facial bone primordium Absent
TS22: cranium Absent Expression was not detected in the skull (base and vault).
TS22: axial skeleton Absent
TS22: tail Weak Ubiquitous Embryo_C2401_4_4D
TS22: forelimb Moderate Regionally restricted Embryo_C2401_4_2B
TS22: hindlimb Moderate Regionally restricted Embryo_C2401_4_2B
TS22: integumental system Moderate Regionally restricted Embryo_C2401_4_2B
TS22: skin Moderate Regionally restricted Embryo_C2401_4_2B
TS22: hair Absent
TS22: vibrissa follicle Absent Expression was not detected in whisker follicle.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory