Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:121435
Samokhvalov IM, et al., Cell tracing shows the contribution of the yolk sac to adult haematopoiesis. Nature. 2007 Apr 26;446(7139):1056-61
|
|
Assay type:
|
Recombinase reporter
|
|
MGI Accession ID:
|
MGI:5316128
|
|
Gene symbol:
|
Runx1
|
|
Gene name:
|
runt related transcription factor 1
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
Assay notes:
|
Tamoxifen was administered to pregnant mice during gestation at embryonic age specified in note, and embryos were assayed at age indicated.
|
|
|
Results
|
Specimen
Fig. 1 E6.5 right:
embryonic day 11.5-12.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more  )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E6.5. Embryos were isolated and stained on E11.5-12.0.
Specimen
Fig. 1 E6.5 right:
(close  )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 11.5-12.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E6.5. Embryos were isolated and stained on E11.5-12.0.
|
Specimen
not shown:
embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E6.5. Embryos were isolated and stained on E11.5-12.0.
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E6.5. Embryos were isolated and stained on E11.5-12.0.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: embryo |
Ambiguous |
|
|
No or very few stained blood cells are observed with tamoxifen treatment at E6.5.
|
| TS19: yolk sac |
Present |
Spotted |
|
Some yolk sacs (2/16) contain clusters of stained visceral endodermal and endothelial cells and a few primitive blood cells.
|
Specimen
Fig. 1 E7.5 right:
embryonic day 11.5-12.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos were isolated and stained on E11.5-12.0.
Specimen
Fig. 1 E7.5 right:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 11.5-12.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos were isolated and stained on E11.5-12.0.
|
Specimen
Fig. 1 E7.5 right inset I:
embryonic day 11.5-12.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos were isolated and stained on E11.5-12.0.
Specimen
Fig. 1 E7.5 right inset I:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 11.5-12.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos were isolated and stained on E11.5-12.0.
|
Specimen
Fig. 1 E7.5 right inset II:
embryonic day 11.5-12.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos and maternal tissue were isolated and stained on E11.5-12.0.
Specimen
Fig. 1 E7.5 right inset II:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 11.5-12.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos and maternal tissue were isolated and stained on E11.5-12.0.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: liver sinusoid |
Present |
|
1_E7.5 induced--II
|
Staining is observed in blood cells accumulating in the hepatic sinusoids with tamoxifen treatment at E7.5.
|
Specimen
Fig. 1 E8.5 right:
embryonic day 11.5-12.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.5. Embryos were isolated and stained on E11.5-12.0.
Specimen
Fig. 1 E8.5 right:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 11.5-12.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.5. Embryos were isolated and stained on E11.5-12.0.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: blood |
Present |
|
1_E8.5 induced
|
Staining is observed in fewer circulating blood cells when tamoxifen is administered at E8.5 versus E7.5.
|
| TS19: liver sinusoid |
Present |
|
1_E8.5 induced
|
Staining is observed in fewer blood cells in the liver when tamoxifen is administered at E8.5 versus E7.5.
|
Specimen
Fig. 1 E9.5 right:
embryonic day 11.5-12.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E9.5. Embryos were isolated and stained on E11.5-12.0.
Specimen
Fig. 1 E9.5 right:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 11.5-12.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E9.5. Embryos were isolated and stained on E11.5-12.0.
|
Specimen
Fig. 1 E9.5 right inset V:
embryonic day 11.5-12.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E9.5. Embryos were isolated and stained on E11.5-12.0.
Specimen
Fig. 1 E9.5 right inset V:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 11.5-12.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E9.5. Embryos were isolated and stained on E11.5-12.0.
|
Specimen
Fig. 2a:
embryonic day 7.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Concepti were isolated and stained 4 hours after injection.
Specimen
Fig. 2a:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Concepti were isolated and stained 4 hours after injection.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Trace |
|
2a
|
Some stained cells are observed in some embryos at 4 hours after tamoxifen treatment.
|
Specimen
Fig. 2b:
embryonic day 7.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Concepti were isolated and stained 8 hours after injection.
Specimen
Fig. 2b:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Concepti were isolated and stained 8 hours after injection.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
|
2b
|
Staining is observed in more embryos at 6 hours after tamoxifen treatment.
|
| TS11: blood island |
Present |
Spotted |
2b
|
Staining is observed in more cells in the blood-island region at 6 hours after tamoxifen treatment.
|
Specimen
Fig. 2c:
embryonic day 8.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Concepti were isolated and stained 12 hours after injection.
Specimen
Fig. 2c:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Concepti were isolated and stained 12 hours after injection.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: yolk sac |
Present |
Regionally restricted |
2c
|
Staining is observed in a band of yolk sac cells in all embryos at 12 hours after tamoxifen treatment.
|
Specimen
Fig. 2f:
embryonic day 8.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Concepti were isolated and stained 12 hours after injection.
Specimen
Fig. 2f:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Concepti were isolated and stained 12 hours after injection.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: yolk sac |
Present |
Regionally restricted |
2f
|
Staining is observed exclusively in the proximal band of yolk sac cells adjacent to the extraembryonic ectoderm.
|
Specimen
Fig. 4a:
embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos and maternal tissue were isolated and stained on E10.5-11.5.
Specimen
Fig. 4a:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos and maternal tissue were isolated and stained on E10.5-11.5.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: umbilical vein |
Present |
|
4a
|
Staining is observed in cells in the endothelium of the umbilical vein with tamoxifen treatment at E7.5.
|
Specimen
Fig. 4b:
embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos and maternal tissue were isolated and stained on E10.5-11.5.
Specimen
Fig. 4b:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos and maternal tissue were isolated and stained on E10.5-11.5.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: umbilical artery |
Present |
|
4b
|
Staining is observed in cells in the endothelium of the umbilical artery with tamoxifen treatment at E7.5.
|
Specimen
Fig. 4c:
embryonic day 10.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos and maternal tissue were isolated and stained on E10.5-11.5.
Specimen
Fig. 4c:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos and maternal tissue were isolated and stained on E10.5-11.5.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dorsal aorta |
Present |
|
4c
|
Staining is observed in cells in the endothelium of dorsal aorta with tamoxifen treatment at E7.5.
|
Specimen
Fig. S4A b:
embryonic day 9.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos were isolated and stained on E9.5.
Specimen
Fig. S4A b:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos were isolated and stained on E9.5.
|
Specimen
Fig. S4A c right embryo:
embryonic day 10.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos and yolk sacs were isolated and stained on E10.5.
Specimen
Fig. S4A c right embryo:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E7.5. Embryos and yolk sacs were isolated and stained on E10.5.
|
Specimen
Fig. S5a:
embryonic day 8.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
Specimen
Fig. S5a:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac |
Present |
Spotted |
S5a
|
Staining is observed only in the yolk sac at 12 hours after tamoxifen treatment at E8.0.
|
Specimen
Fig. S5b:
embryonic day 8.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
Specimen
Fig. S5b:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac |
Present |
Spotted |
S5b
|
Staining is observed only in the yolk sac at 12 hours after tamoxifen treatment at E8.0.
|
Specimen
Fig. S5c:
embryonic day 8.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
Specimen
Fig. S5c:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac |
Present |
Spotted |
S5c
|
Staining is observed only in the yolk sac at 12 hours after tamoxifen treatment at E8.0.
|
Specimen
Fig. S5d:
embryonic day 8.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
Specimen
Fig. S5d:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac |
Present |
Spotted |
S5d
|
Staining is observed only in the yolk sac vasculature at 12 hours after tamoxifen treatment at E8.0.
|
| TS13: embryo |
Absent |
|
S5d
|
No staining of intraembryonic cells from the caudal region is observed.
|
Specimen
Fig. S5e:
embryonic day 8.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
Specimen
Fig. S5e:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
|
Specimen
Fig. S5f:
embryonic day 8.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
Specimen
Fig. S5f:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
|
Specimen
Fig. S5g:
embryonic day 8.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Runx1tm1(cre/Esr1*)Ims/Runx1 +
(more )
Note: Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
Specimen
Fig. S5g:
(close )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Runx1tm1(cre/Esr1*)Ims/Runx1+
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Conditional mutant. 5-10 mg of 4-hydroxytamoxifen (4-OHT) in sunflower oil was administered to pregnant mice by intraperitoneal injection at E8.0. Embryos were isolated and stained 12 hours later.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: allantois |
Absent |
|
S5g
|
No staining is observed in the allantois or embryo.
|
| TS13: embryo |
Absent |
|
S5g
|
No staining is observed in the allantois or embryo.
|
|
Analysis Tools