Probe:	Mirg cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		2A

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Strong		2B
TS15: future midbrain	Strong		2B

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Strong		2C

Structure	Level	Pattern	Image	Note
TS19: inner ear	Present	Regionally restricted	2E	Expression was detected in the otic vesicle.
TS19: branchial arch	Present		2E
TS19: forelimb bud	Present		2E
TS19: hindlimb bud	Present		2E
TS19: somite	Present		2E

Structure	Level	Pattern	Image	Note
TS19: inner ear	Present	Regionally restricted	2F	Expression was detected in the otic vesicle.
TS19: forelimb bud	Present		2F
TS19: somite	Present		2F

Structure	Level	Pattern	Image	Note
TS21: forebrain	Strong		3A
TS21: hindbrain	Strong		3A
TS21: cerebral cortex	Present		3C , 3A
TS21: telencephalon	Present	Regionally restricted	3A , 3C	High expression was detected in the basal ganglia.
TS21: midbrain	Strong	Regionally restricted	3A , 3D	Expression was detected in the midbrain cortex.
TS21: cerebellum	Present		3D , 3A
TS21: medulla oblongata	Present		3E , 3A
TS21: remnant of Rathke's pouch	Present		3A , 3F
TS21: central nervous system ganglion	Present	Regionally restricted	3A , 3F	High expression was detected in the nerve ganglion.
TS21: tongue	Strong		3G , 3A
TS21: heart	Weak		3H , 3A
TS21: lung	Weak		3I , 3A
TS21: liver	Weak		3A , 3J

Structure	Level	Pattern	Image	Note
TS23: nervous system	Present		4A	Prominent expression was observed in neural tissues.
TS23: skeletal muscle	Present		4A
TS23: diaphragm	Present	Regionally restricted	4A	Expression was detected in diaphragmatic muscle.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Strong		4B' , 4B	Expression appeared to be in the nuclei of the nerve cells.
TS23: cerebellum	Strong		4B

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		4C
TS23: corpus striatum	Present		4C
TS23: main olfactory bulb	Present		4C

Structure	Level	Pattern	Image	Note
TS23: pituitary gland	Present		4D , 4D'	Expression appeared to be in the nuclei and the cytoplasm.

Structure	Level	Pattern	Image	Note
TS23: tongue	Present		4E' , 4E	Expression appeared to be in the nuclei of the developing muscle.

Structure	Level	Pattern	Image	Note
TS23: axial muscle	Present	Regionally restricted	4F' , 4F	Expression was detected in back muscle. Expression appeared to be in the nuclei of the developing muscle.
TS23: erector spinae	Present		4F

Structure	Level	Pattern	Image	Note
TS23: intercostal muscle	Present		4G' , 4G	Expression appeared to be in the nuclei of the developing muscle.

Structure	Level	Pattern	Image	Note
TS23: cartilage	Weak		4H' , 4H	Expression appeared to be in the nuclei and the cytoplasm.

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Present		4I' , 4I	Expression appeared to be in the nuclei and the cytoplasm.

Structure	Level	Pattern	Image	Note
TS23: heart	Absent		4J

Structure	Level	Pattern	Image	Note
TS23: lung	Absent		4K

Structure	Level	Pattern	Image	Note
TS23: liver	Absent		4L

Structure	Level	Pattern	Image	Note
TS23: pancreas	Weak		4M

Structure	Level	Pattern	Image	Note
TS23: metanephros	Weak		4N