Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:180802
Curlin M, et al., Stam2 expression pattern during embryo development. Gene Expr Patterns. 2012 Jan;12(1-2):68-76
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:5314385
|
Gene symbol:
|
Stam2
|
Gene name:
|
signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
|
Antibody:
|
anti-Stam2
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
Assay notes:
|
Expression levels in the table were assigned based on the descriptions in the text (+/-, weak; +, present; ++, strong and +++, very strong).
|
|
Results
|
Specimen
4D left:
embryonic day 16.5; Stam2Gt(pKC199)1Gaj/Stam2 +
(more )
Note: Double labeled: red - Stam2 protein; red - Stam2 reporter (assay MGI:5313534).
Specimen
4D left:
(close )
Genetic Background:
|
B6NCrl.Cg-Stam2Gt(pKC199)1Gaj
|
Mutant Allele(s):
|
Stam2Gt(pKC199)1Gaj/Stam2+
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Stam2 protein; red - Stam2 reporter (assay MGI:5313534).
|
Specimen
4E left:
embryonic day 11.5; Stam2Gt(pKC199)1Gaj/Stam2 +
(more )
Note: Double labeled: red - Stam2 protein; red - Stam2 reporter (assay MGI:5313534).
Specimen
4E left:
(close )
Genetic Background:
|
B6NCrl.Cg-Stam2Gt(pKC199)1Gaj
|
Mutant Allele(s):
|
Stam2Gt(pKC199)1Gaj/Stam2+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Stam2 protein; red - Stam2 reporter (assay MGI:5313534).
|
Specimen
Table2 E11.5:
(close )
Genetic Background:
|
B6NCrl.Cg-Stam2Gt(pKC199)1Gaj
|
Mutant Allele(s):
|
Stam2Gt(pKC199)1Gaj/Stam2+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Table2 E16.5:
(close )
Genetic Background:
|
B6NCrl.Cg-Stam2Gt(pKC199)1Gaj
|
Mutant Allele(s):
|
Stam2Gt(pKC199)1Gaj/Stam2+
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
(close )
Genetic Background:
|
B6NCrl.Cg-Stam2Gt(pKC199)1Gaj
|
Mutant Allele(s):
|
Stam2Gt(pKC199)1Gaj/Stam2+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube |
Present |
Widespread |
|
Present throughout the whole section, with strong expression in the ventral part.
|
|