Gene Expression Data
Assay Details
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Assay
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Reference:
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J:179032
Sharlin DS, et al., Developmental and cell-specific expression of thyroid hormone transporters in the mouse cochlea. Endocrinology. 2011 Dec;152(12):5053-64
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5308803
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Gene symbol:
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Slc7a5
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Gene name:
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solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
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Probe:
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Lat1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A E18:
embryonic day 18.0
(more )
Specimen
1A E18:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlear ganglion |
Weak |
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1A E18
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| TS26: cochlear duct |
Weak |
Regionally restricted |
1A E18
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Signals were detected in the spiral limbus, spiral ligament, and stria vascularis in a pattern indicative of sectioned blood vessels. Signal was also detected in spiral vessel, a blood vessel below the organ of Corti.
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Specimen
1A P3:
postnatal day 3
(more )
Specimen
1A P3:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlear ganglion |
Present |
Regionally restricted |
1A P3
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Weaker signals were detected in nonvascular cell types in the spiral ganglion.
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| TS27: spiral ligament |
Present |
Regionally restricted |
1A P3
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Staining was in a pattern indicative of sectioned blood vessel.
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| TS27: spiral limbus |
Present |
Regionally restricted |
1A P3
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Staining was in a pattern indicative of sectioned blood vessel.
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| TS27: stria vascularis |
Present |
Regionally restricted |
1A P3
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Staining was in a pattern indicative of sectioned blood vessel.
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| TS27: organ of Corti |
Present |
Regionally restricted |
1A P3
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Weaker signals were detected in the greater and lesser epithelial ridges and hair cells of the organ of Corti.
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| TS27: spiral vessel |
Present |
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1A P3
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Signal was detected in the spiral vessel, a blood vessel below the organ of Corti.
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Specimen
1A P5:
postnatal day 5
(more )
Specimen
1A P5:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1A P7:
postnatal day 7
(more )
Specimen
1A P7:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlear ganglion |
Present |
Regionally restricted |
1A P7
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Staining was in a pattern indicative of sectioned blood vessel.
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| TS28: spiral ligament |
Present |
Regionally restricted |
1A P7
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Staining was in a pattern indicative of sectioned blood vessel.
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| TS28: spiral limbus |
Present |
Regionally restricted |
1A P7
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Staining was in a pattern indicative of sectioned blood vessel.
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| TS28: stria vascularis |
Present |
Regionally restricted |
1A P7
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Staining was in a pattern indicative of sectioned blood vessel.
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| TS28: spiral vessel |
Present |
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1A P7
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Specimen
1A P10:
postnatal day 10
(more )
Specimen
1A P10:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlear ganglion |
Present |
Regionally restricted |
1A P10
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Staining was in a pattern indicative of sectioned blood vessel. Expression signals decreased after P7.
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| TS28: spiral ligament |
Present |
Regionally restricted |
1A P10
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Staining was in a pattern indicative of sectioned blood vessel. Expression signals decreased after P7.
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| TS28: spiral limbus |
Present |
Regionally restricted |
1A P10
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Staining was in a pattern indicative of sectioned blood vessel. Expression signals decreased after P7.
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| TS28: stria vascularis |
Present |
Regionally restricted |
1A P10
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Staining was in a pattern indicative of sectioned blood vessel. Expression signals decreased after P7.
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| TS28: spiral vessel |
Present |
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1A P10
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Expression signals decreased after P7.
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Specimen
3A:
postnatal day 7
(more )
Specimen
3A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: stria vascularis |
Present |
Regionally restricted |
3A
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Signal in the intermediate region of stria vascularis.
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| TS28: spiral ligament |
Present |
Regionally restricted |
3A
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Signals in blood vessel-like structures in the spiral ligament.
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Specimen
3C:
postnatal day 3
(more )
Specimen
3C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
3C
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Signals were transiently detected in inner and outer hair cells and more weakly in Deiters support cells residing below the outer hair cells.
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| TS27: cochlear duct |
Present |
Regionally restricted |
3C
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Signal was detected in the spiral vessel.
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Specimen
3E:
postnatal day 1
(more )
Specimen
3E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlear ganglion |
Present |
Regionally restricted |
3E
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Signals were in vessel-like structures and weakly in cell bodies of neurons.
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| TS27: blood vessel |
Present |
Regionally restricted |
3E
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Signal was detected in bood vessels of the spiral ganglion.
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Specimen
3G:
postnatal day 7
(more )
Specimen
3G:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6A Tshr+/+:
postnatal day 7
(more )
Specimen
6A Tshr<+/+>:
(close )
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Genetic Background:
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involves: 129 * 129S1/Sv * C57BL/6J
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6A Tsgr<-/->:
(close )
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Genetic Background:
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involves: 129 * 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Tshrtm1Rmar/Tshrtm1Rmar
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
Present |
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6A Tshr-/-
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Expression was unchanged in mutant mice compared with wild type.
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Specimen
S1A Lat1:
postnatal day 7
(more )
Specimen
S1A Lat1:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
Present |
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S1A Lat1
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