About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:178893 Sivakamasundari V, et al., New Bapx1(Cre-EGFP) mouse lines for lineage tracing and conditional knockout studies. Genesis. 2012 Apr;50(4):375-83
Assay type: Recombinase reporter
MGI Accession ID: MGI:5308210
Gene symbol: Nkx3-2
Gene name: NK3 homeobox 2
Reporter: lacZ
Direct Detection
Results
Specimen Fig. 2A b: embryonic day 9.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: head mesenchyme Present 2A_b Staining is observed in the cephalic mesenchyme.
TS15: mesenchyme derived from splanchnopleure Present 2A_b Staining is observed in the splanchnic mesenchyme.
TS15: somite Present 2A_b Staining is observed in the somites.
Specimen Fig. 2A e: embryonic day 10.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: head mesenchyme Present 2A_e
TS17: sclerotome Present 2A_e
TS17: trunk mesenchyme Present 2A_e
TS17: 1st branchial arch Weak 2A_e Staining is observed around the first branchial arch.
Specimen Fig. 2A h: embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present 2A_h
TS19: vertebral pre-cartilage condensation Present 2A_h
TS19: 1st branchial arch Present 2A_h Staining is stronger in the first arch than that observed at E10.5.
Specimen Fig. 2A k: embryonic day 12.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: Meckel's cartilage Present 2A_k Staining is observed in a domain adjoining Meckel's cartilage.
TS20: rib pre-cartilage condensation Present 2A_k Staining persists in the cartilage condensations of the ribs.
TS20: forelimb pre-cartilage condensation Present 2A_k Staining persists in the cartilage condensations of the forelimbs.
TS20: vertebral cartilage condensation Present 2A_k Staining persists in the cartilage condensations of the vertebra.
TS20: hindlimb pre-cartilage condensation Present 2A_k Staining is observed in the hindlimbs for the first time.
Specimen Fig. 2A n: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb pre-cartilage condensation Present 2An
TS21: limb pre-cartilage condensation Present 2An
TS21: vertebral cartilage condensation Present 2An
TS21: rib pre-cartilage condensation Present 2An
Specimen Fig. 2B top left: embryonic day 12.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: forelimb pre-cartilage condensation Present 2B_top left Staining is observed in the cartilage condensations of the forelimb.
Specimen Fig. 2B top right: embryonic day 12.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: hindlimb pre-cartilage condensation Present 2B_top right Staining is observed in the cartilage condensations of hindlimb (stylopod, zeugopod and autopod regions).
Specimen Fig. 2B middle left: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb pre-cartilage condensation Present 2B_middle left Staining is observed in the segments of the forelimb.
TS21: shoulder mesenchyme Present 2B_middle left Staining is observed in the scapula.
TS21: radius-ulna cartilage condensation Present 2B_middle left Staining is observed in the radius and ulna.
TS21: humerus cartilage condensation Present 2B_middle left Staining is observed in the humerus.
TS21: metacarpus pre-cartilage condensation Present 2B_middle left Staining is observed in the future metacarpals.
TS21: forelimb digit pre-cartilage condensation Present 2B_middle left Staining is observed in the digits.
Specimen Fig. 2B middle right: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: femur cartilage condensation Present 2B_middle right Staining is observed in the stylopod, zeugopod, and autopod regions of the hindlimb.
TS21: foot pre-cartilage condensation Present 2B_middle right Staining is observed in the stylopod, zeugopod, and autopod regions of the hindlimb.
TS21: hindlimb pre-cartilage condensation Present 2B_middle right Staining is observed in the stylopod, zeugopod, and autopod regions of the hindlimb.
TS21: tibia-fibula pre-cartilage condensation Present 2B_middle right Staining is observed in the stylopod, zeugopod, and autopod regions of the hindlimb.
Specimen Fig. 2B bottom left: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: shoulder Present 2B_bottom left Staining is observed in the scapula.
TS23: radius Present 2B_bottom left Staining is observed in the radius and ulna.
TS23: ulna Present 2B_bottom left Staining is observed in the radius and ulna.
TS23: humerus Present 2B_bottom left Staining is observed in the humerus.
TS23: metacarpus Present 2B_bottom left Staining is observed in the future metacarpals.
TS23: forelimb digit Present 2B_bottom left Staining is observed in the digits.
Specimen Fig. 2B bottom right: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: hindlimb mesenchyme Present 2B_bottom right Staining is observed in the stylopod, zeugopod, and autopod regions of the hindlimb.
Specimen Fig. 3b: embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: vertebral pre-cartilage condensation Present 3b Staining is observed in condensing mesenchymal prevertebrae.
TS19: neural tube Absent 3b No staining is observed in neural tube tissue.
Specimen Fig. 3e: embryonic day 12.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: intestine mesenchyme Present 3e Staining is observed in the intestinal mesoderm.
TS20: intestine epithelium Absent 3e No staining is observed in the endodermal epithelium of the intestine.
Specimen Fig. 3h: embryonic day 12.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: forelimb pre-cartilage condensation Present 3h , 3k Staining is observed in the cartilage condensations of the limbs.
Specimen Fig. 5b: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: dorsal mesogastrium Present Regionally restricted 5e , 5b Staining is observed in the splenic mesenchyme.
TS21: pancreas Ambiguous 5b , 5e Staining is reported to be observed in the dorsal pancreatic tissue.
TS21: stomach epithelium Present 5e , 5b Staining is observed in the stomach epithelium.
Specimen Fig. 5i: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: dorsal mesogastrium Present Regionally restricted 5j , 5i Staining is observed in the splenic mesenchyme.
TS21: pancreas Ambiguous 5j , 5i Staining is reported to be observed in the dorsal pancreatic tissue.
TS21: stomach epithelium Present 5j , 5i Staining is observed in the stomach epithelium.
Specimen Fig. 5l: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-2tm3.1(cre)Tlu/Nkx3-2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: dorsal mesogastrium Present 5l Staining is observed in the splenic mesenchyme.
TS21: pancreas Ambiguous 5l Staining is reported to be observed in the dorsal pancreatic tissue.
TS21: stomach epithelium Ambiguous 5l Staining is observed in the stomach epithelium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory