Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:57297
St-Jacques B, et al., Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation [published erratum appears in Genes Dev 1999 Oct 1;13(19):2617]. Genes Dev. 1999 Aug 15;13(16):2072-86
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5302044
|
|
Gene symbol:
|
Bmp3
|
|
Gene name:
|
bone morphogenetic protein 3
|
|
Probe:
|
Bmp3 probe3
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
not shown WT:
embryonic day 13.5
(more  )
Specimen
not shown WT:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: upper arm mesenchyme |
Present |
Regionally restricted |
|
Expression is present in the perichondrium/periosteum.
|
Specimen
8E:
embryonic day 14.5
(more )
Specimen
8E:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
8F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Ihhtm1Amc/Ihhtm1Amc
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
8G:
embryonic day 18.5
(more )
Specimen
8G:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: humerus |
Present |
|
8G
|
Expression is present in areas of trabecular and cortical bone formation, where preosteoblast and osteoblast differentiation has taken place.
|
Specimen
8H:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Ihhtm1Amc/Ihhtm1Amc
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: humerus |
Present |
Regionally restricted |
8H
|
Some expression is present at the core of the humerus.
|
| TS26: upper arm mesenchyme |
Absent |
|
8H
|
There is no expression in the perichondrial/periosteal region.
|
|
Analysis Tools