Gene Expression Data
Assay Details
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Assay
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Reference:
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J:57297
St-Jacques B, et al., Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation [published erratum appears in Genes Dev 1999 Oct 1;13(19):2617]. Genes Dev. 1999 Aug 15;13(16):2072-86
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5302041
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Gene symbol:
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Runx2
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Gene name:
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runt related transcription factor 2
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Probe:
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Cbfa1 cDNA7
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
8A:
embryonic day 13.5
(more  )
Specimen
8A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
8B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: humerus cartilage condensation |
Present |
Regionally restricted |
8B
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Expression is present only in the more mature chondrocytes at the center of the humerus. It is not present in the perichondrium.
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Specimen
8C:
embryonic day 18.5
(more )
Specimen
8C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: humerus |
Strong |
Non-Uniform |
8C
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Strong expression is present in the osteogenic areas and the trabecular bone; it is expressed at low levels in the chondrocytes.
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| TS26: upper arm mesenchyme |
Strong |
Regionally restricted |
8C
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Expression is present in the periosteum of the humerus.
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Specimen
8D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: humerus |
Present |
Regionally restricted |
8D
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Expression is present in the chondrocytes.
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| TS26: upper arm mesenchyme |
Absent |
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8D
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There is no expression in the perichondrial/periosteal tissue.
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Analysis Tools