Probe:	Phox2b cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		2I
TS17: rhombomere 2	Present	Non-Uniform	2I	Expression is upregulated in a medial column extending from rhombomere 2 to 6.
TS17: rhombomere 3	Present	Non-Uniform	2I	Expression is upregulated in a medial column extending from rhombomere 2 to 6.
TS17: rhombomere 4	Present	Non-Uniform	2I	Expression is strongest in a ventral rhombomere 4 column. It is also present in a dorsal column extending from rhombomere 4 posteriorly. The medial and dorsal columns overlap in rhombomeres 4, 5 and 6.
TS17: rhombomere 5	Present	Non-Uniform	2I	Expression is upregulated in a medial column extending from rhombomere 2 to 6. It is also present in a dorsal column extending from rhombomere 4 posteriorly. The medial and dorsal columns overlap in rhombomeres 4, 5 and 6.
TS17: rhombomere 6	Present	Non-Uniform	2I	Expression is upregulated in a medial column extending from rhombomere 2 to 6. It is also present in a dorsal column extending from rhombomere 4 posteriorly. The medial and dorsal columns overlap in rhombomeres 4, 5 and 6.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		2J	Expression is present in the mutant, but unlike in wild-type, the medial and dorsal columns overlap by only two rhombomeres.
TS17: rhombomere 4	Present		2J	In the mutant there is no rhombomere 4 specific upregulation of expression, leaving it similar to that found in rhombomeres 2 and 3.
TS17: rhombomere 2	Present		2J
TS17: rhombomere 3	Present		2J

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Non-Uniform	2K
TS17: rhombomere 4	Present	Non-Uniform	2K	Expression levels in ventral rhombomere 4 are lower than in wild type but remain elevated when compared with neighboring rhombomeres.
TS17: rhombomere 3	Present		2K
TS17: rhombomere 5	Present		2K

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		2L	Expression is present in the mutant, but there is no rhombomere 4 specific upregulation of expression. Unlike in wild-type, the medial and dorsal columns overlap by only one rhombomere.
TS17: rhombomere 4	Present		2L	Expression is present in the mutant, but there is no rhombomere 4 specific upregulation of expression. Unlike in wild-type, the medial and dorsal columns overlap by only one rhombomere.

Structure	Level	Pattern	Image	Note
TS20: facial ganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS20: facial ganglion	Absent		5B	There is a lack of expression in the migratory pathway of the facial motoneurons.

Structure	Level	Pattern	Image	Note
TS20: facial ganglion	Present		5C

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Present		5D
TS20: facial ganglion	Present		5D

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Present		5E
TS20: facial ganglion	Absent		5E	There is a lack of expression in the migratory pathway of the facial motoneurons.
TS20: cranial ganglion	Present	Non-Uniform	5E	Expression is present in ectopic nuclei.

Structure	Level	Pattern	Image	Note
TS20: facial ganglion	Present		5F
TS20: trigeminal ganglion	Present		5F
TS20: cranial ganglion	Present	Non-Uniform	5F	Expression is present in ectopic nuclei.