Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79856
Rallu M, et al., Dorsoventral patterning is established in the telencephalon of mutants lacking both Gli3 and Hedgehog signaling. Development. 2002 Nov;129(21):4963-74
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5298529
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Gene symbol:
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Ascl1
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Gene name:
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achaete-scute family bHLH transcription factor 1
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Probe:
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clone 2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
Not shown:
embryonic day 12.5
(more  )
Specimen
Not shown:
(close  )
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Genetic Background:
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C3HeB/FeJ
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
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Expression is present in the ventral telencephalon.
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
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Expression in the mutant spreads precociously into cortical areas.
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Specimen
4J:
embryonic day 12.5
(more )
Specimen
4J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
4J
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Expression is present in the ventral telencephalon.
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Specimen
4K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6
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Mutant Allele(s):
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Gli3Xt-J/Gli3+
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
4K
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The level of expression and its distribution in the mutants closely resembles that seen in wild-type embryo.
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Analysis Tools
