Wnt3a RNA in situ Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:14717 Parr BA, et al., Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds. Development. 1993 Sep;119(1):247-61
Assay type:	RNA in situ
MGI Accession ID:	MGI:5296977
Gene symbol:	Wnt3a
Gene name:	wingless-type MMTV integration site family, member 3A

Probe:	Wnt3a probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Results

3_3a ©

Specimen 3: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Non-Uniform	3_3a	Expression is primarily in the dorsal region.
TS15: future midbrain	Present	Regionally restricted	3_3a	Expression is present in the dorsal region.

5. Part 1_3a A ©

Specimen 5A: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	5. Part 1_3a A	Expression is confined to a small cluster of cells at the dorsal midline.

5. Part 1_3a B ©

Specimen 5B: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong	Regionally restricted	5. Part 1_3a B	In the rostral diencephalon, expression is confined to dorsal midline cells.

5. Part 1_3a C ©

Specimen 5C: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong	Regionally restricted	5. Part 1_3a C	In the caudal diencephalon, the domain of expression is broader at the level of its dorsal to ventral stripe of expression.

5. Part 2_3a D ©

Specimen 5D: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		5. Part 2_3a D

5. Part 2_3a E ©

Specimen 5E: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		5. Part 2_3a E

5. Part 2_3a F ©

Specimen 5F: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		5. Part 2_3a F
TS15: future hindbrain	Present	Regionally restricted	5. Part 2_3a F	Expression is present n the metencephalon.

8_3a ©

Specimen 8: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Absent		8_3a
TS15: neural tube roof plate	Present	Regionally restricted	8_3a	Expression is present dorsally, in the roof plate region.
TS15: neural tube floor plate	Absent		8_3a

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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