Gene Expression Data
Assay Details
Assay
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Reference:
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J:42455
Dunwoodie SL, et al., Mouse Dll3: a novel divergent Delta gene which may complement the function of other Delta homologues during early pattern formation in the mouse embryo. Development. 1997 Aug;124(16):3065-76
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5295436
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Gene symbol:
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Dll3
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Gene name:
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delta like canonical Notch ligand 3
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Probe:
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Dll3 cDNA2
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Probe preparation:
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Antisense RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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It is possible that Probe Dll3 cDNA3 (MGI:2446514) was used for this assay.
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Results
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Specimen
7A:
embryonic day 9.5
(more )
Specimen
7A:
(close )
Genetic Background:
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involves: C57BL/6 * DBA
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Age:
|
embryonic day 9.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7B:
embryonic day 11.5
(more )
Note: Double labeled: red - Dll3; green - BrdU.
Specimen
7B:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA
|
Age:
|
embryonic day 11.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: red - Dll3; green - BrdU.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube lateral wall |
Present |
Regionally restricted |
7D
,
7C
,
7B
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Expression in postmitotic cells lateral to the cells labeled with BrdU along most of the dorsoventral extent. At the region opposite the basal plate, cells expressing Dll3 and cells labelled with BrdU are scattered between the basal and lumenal surface.
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Specimen
7E:
embryonic day 11.0
(more )
Note: Double labeled: red - Dll3; green - Isl1 (assay MGI:5295451) and Isl2 (assay MGI:5295452).
Specimen
7E:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Dll3; green - Isl1 (assay MGI:5295451) and Isl2 (assay MGI:5295452).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube ventricular layer |
Present |
Regionally restricted |
7E
,
7F
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Most of the cells that express Dll3 are devoid of the islet-1/2 protein. Coexpression is seen in occasional cells adjacent to the basal plate.
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Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 15.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|