Probe:	Mmp2 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Regionally restricted	4a/b
TS17: 2nd branchial arch	Present	Regionally restricted	4a/b
TS17: 3rd branchial arch	Present	Regionally restricted	4a/b
TS17: branchial arch mesenchyme	Strong		4a/b	Intense expression throughout mesenchymal tissue.
TS17: branchial arch ectoderm	Absent		4a/b	No expression in surface ectoderm or pharyngeal pouch epithelium.
TS17: branchial arch endoderm	Absent		4a/b	No expression in surface ectoderm or pharyngeal pouch epithelium.

Structure	Level	Pattern	Image	Note
TS17: 3rd branchial arch mesenchyme	Strong		4c/d	Intense expression throughout mesenchymal tissue.
TS17: 3rd branchial arch ectoderm	Absent		4c/d	No expression in surface ectoderm or pharyngeal pouch epithelium.
TS17: 3rd branchial pouch endoderm	Absent		4c/d	No expression in surface ectoderm or pharyngeal pouch epithelium.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Strong	Regionally restricted	4e/f	Intense expression throughout mesenchymal tissue.
TS19: 1st branchial pouch endoderm	Absent		4e/f	No expression in pharyngeal pouch epithelium.

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Present	Regionally restricted	5a/b
TS21: lower jaw mesenchyme	Strong	Regionally restricted	5a/b	Intense expression in osteogenic mesenchyme in lower jaw, in cells differentiating to mandibular bone.
TS21: lower jaw skeleton	Strong	Regionally restricted	5a/b	Intense expression in osteogenic mesenchyme in lower jaw, in cells differentiating to mandibular bone.
TS21: mandible mesenchyme	Present	Regionally restricted	5a/b	Uniform expression in mesenchyme of palatal processes, and undifferentiated mesenchyme of mandibular process.
TS21: Meckel's cartilage	Strong	Regionally restricted	5a/b	Intense expression in perichondrial cells around Meckel's cartilage.
TS21: tongue mesenchyme	Present	Regionally restricted	5a/b	Lower expression in differentiating muscle cells in the tongue than in other tongue mesenchyme.
TS21: tongue muscle	Weak		5a/b	Lower expression in differentiating muscle cells in the tongue than in other tongue mesenchyme.
TS21: tongue epithelium	Absent		5a/b
TS21: brain	Absent		5a/b	No expression in neural tissue, either in brain or trigeminal ganglia.
TS21: trigeminal ganglion	Absent		5a/b	No expression in neural tissue, either in brain or trigeminal ganglia.

Structure	Level	Pattern	Image	Note
TS21: eye	Present	Regionally restricted	5c/d	Expression in mesenchymal cells of vitreous body.
TS21: cornea stroma	Present		5c/d
TS21: lens epithelium	Absent		5c/d
TS21: retina epithelium	Absent		5c/d

Structure	Level	Pattern	Image	Note
TS21: lower jaw mesenchyme	Present		5e/f
TS21: tooth epithelium	Absent		5e/f
TS21: submandibular gland primordium epithelium	Present	Regionally restricted	5e/f
TS21: oral epithelium	Absent		5e/f

Structure	Level	Pattern	Image	Note
TS22: tooth epithelium	Absent		6a/b	No expression in epithelium of cap-staged tooth germ.
TS22: dental papilla	Strong		6a/b	Uniform intense expression in dental papilla mesenchyme and surrounding jaw mesenchyme.
TS22: jaw mesenchyme	Strong		6a/b	Uniform intense expression in dental papilla mesenchyme and surrounding jaw mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: salivary gland epithelium	Strong		6c/d
TS22: salivary gland mesenchyme	Weak		6c/d

Structure	Level	Pattern	Image	Note
TS22: upper lip	Strong	Regionally restricted	6e/f	Intense expression in deeper mesenchymal cell layers.

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present	Regionally restricted	6g	Expression in central epithelial cells of hair bud.

Structure	Level	Pattern	Image	Note
TS22: mesenchyme of metanephros	Present		7a/b	Expression in all kidney mesenchymal cells.
TS22: metanephros	Present	Regionally restricted	7a/b	No expression in kidney epithelia.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present	Regionally restricted	7c	Intense expression in periurethral cells may be due to high cell density.

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Weak	Regionally restricted		Very weak expression in epithelial cells of differentiating kidney tubules.

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present		7d/e	Expression in all lung mesenchymal cells.
TS22: lung epithelium	Absent		7d/e

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present		7f	Expression in all lung mesenchymal cells.
TS22: lung epithelium	Absent		7f

Structure	Level	Pattern	Image	Note
TS22: pancreas mesenchyme	Present
TS22: pancreas epithelium	Absent

Structure	Level	Pattern	Image	Note
TS23: hand mesenchyme	Present	Regionally restricted	7g/h	Expression in loose mesenchyme as well as in chondrocytes.
TS23: forelimb interdigital region mesenchyme	Absent		7g/h	Negative expression in areas between cartilages.
TS23: hand skin	Absent		7g/h	No expression in interdigital epithelial tissue or in surface epithelium.

Structure	Level	Pattern	Image	Note
TS28: renal medulla	Present	Regionally restricted	8a/b	Expression in medullary as well as interpyramidal stroma.

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Absent		8c/d	No expression in myocardial muscle cells.
TS28: heart	Strong	Regionally restricted	8c/d	Intense expression in single cells lining myocardial cell bundles.

Structure	Level	Pattern	Image	Note
TS28: bronchus epithelium	Absent		8e/f	No expression in bronchial epithelium.
TS28: bronchus	Strong	Regionally restricted	8e/f	Intense expression in mesenchymal cells in close association with the epithelium.

Structure	Level	Pattern	Image	Note
TS28: bronchus epithelium	Absent		8g	No expression in bronchial epithelium.
TS28: bronchus	Strong	Regionally restricted	8g	Intense expression in mesenchymal cells in close association with the epithelium.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Regionally restricted	8h	Expression in mesenchymal cells surrounding bile ducts.
TS28: liver parenchyma	Absent		8h	No expression in hepatocytes of the parenchyma.

Structure	Level	Pattern	Image	Note
TS28: epidermis	Absent		8i	No expression in skin epithelium.
TS28: hair follicle	Present	Regionally restricted	8i	Occasional expression in inner cells of hair follicles.
TS28: dermis	Strong	Regionally restricted	8i	Intense expression in stromal fibroblasts.