Gene Expression Data
Assay Details
Assay
|
Reference:
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J:52209
Serpinskaya AS, et al., Synapse formation by hippocampal neurons from agrin- deficient mice. Dev Biol. 1999 JAN 1;205(1):65-78
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5287898
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Gene symbol:
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Dlg4
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Gene name:
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discs large MAGUK scaffold protein 4
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Antibody:
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anti-PSD-95
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Detection system:
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Secondary antibody
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Results
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Specimen
2b:
postnatal adult
(more )
Specimen
2b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
Present |
Punctate |
2b
|
Expression is concentrated at synapses.
|
Specimen
2b':
postnatal adult
(more )
Note: Also stained for Sv2a (assay MGI:5287885) and Sv2b (assay MGI:5287893).
Specimen
2b':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Also stained for Sv2a (assay MGI:5287885) and Sv2b (assay MGI:5287893).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
Present |
Punctate |
2b'
|
Expression is concentrated at synapses.
|
Specimen
2h:
embryonic day 18.0; Agrntm1Jrs/Agrn +
(more )
Specimen
2h:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
|
Agrntm1Jrs/Agrn+
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Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: hippocampus |
Present |
Punctate |
2h
|
Expression is concentrated at synapses.
|
Specimen
2h':
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Agrntm1Jrs/Agrn+
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Also stained for Sv2a (assay MGI:5287885) and Sv2b (assay MGI:5287893).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: hippocampus |
Present |
Punctate |
2h'
|
Expression is concentrated at synapses.
|
Specimen
2n:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Agrntm1Jrs/Agrntm1Jrs
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: hippocampus |
Present |
Punctate |
2n
|
Expression is concentrated at synapses and is the same as in wild-type.
|
Specimen
2n':
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Agrntm1Jrs/Agrntm1Jrs
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Also stained for Sv2a (assay MGI:5287885) and Sv2b (assay MGI:5287893).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: hippocampus |
Present |
Punctate |
2n'
|
Expression is concentrated at synapses and is the same as in wild-type.
|
|