Gene Expression Data
Assay Details
Assay
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Reference:
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J:162481
Hoesel B, et al., Combination of in silico and insitu hybridisation approaches to identify potential Dll1 associated miRNAs during mouse embryogenesis. Gene Expr Patterns. 2010 Sep;10(6):265-73
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5287476
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Gene symbol:
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Mir449a
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Gene name:
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microRNA 449a
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Probe:
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miR-449a probe
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Probe preparation:
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Antisense labelled with digoxigenin DNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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A single-DIG labeled probe was used for these analyses.
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Results
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Specimen
Not shown:
embryonic day 9.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
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C3HeB/FeJ
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: embryo |
Absent |
|
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The only staining seen was unspecific staining in the neural tube.
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Specimen
4J:
embryonic day 10.5
(more )
Specimen
4J:
(close )
Genetic Background:
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C3HeB/FeJ
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Age:
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embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS17: hindbrain |
Present |
Regionally restricted |
4J-left
,
4J-right
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Expression is restricted to a V-shaped domain in the dorsal-most aspect of the rhombencephalon. It is present in a salt and pepper pattern in the dorsal aspect of the rhombic lip and its caudal limit is at the hindbrain-spinal cord junction.
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