Gene Expression Data
Assay Details
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Assay
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Reference:
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J:142123
Ishimura A, et al., Man1, an inner nuclear membrane protein, regulates left-right axis formation by controlling nodal signaling in a node-independent manner. Dev Dyn. 2008 Dec;237(12):3565-76
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5287250
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Gene symbol:
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Lefty1
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Gene name:
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left right determination factor 1
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Probe:
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Lefty1 probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3A:
embryonic day 8.0
(more  )
Note: 4 somite stage.
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural plate |
Present |
Regionally restricted |
3A
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Expression was detected in the prospective floor plate.
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| TS12: lateral plate mesoderm |
Weak |
Regionally restricted |
3A
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Weak expression was detected in the left anterior lateral plate mesoderm.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
3B
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Expression was elevated markedly in the midline.
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| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3B
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Expression was elevated in the left lateral plate mesoderm and extended more posteriorly on the left than in wild type. Ectopic expression was detected in the anterior half of the right lateral plate mesoderm.
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Specimen
3C:
embryonic day 8.0
(more )
Note: 4 somite stage. Transverse section of the embryo in Fig. 3A.
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Note:
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4 somite stage. Transverse section of the embryo in Fig. 3A.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural plate |
Present |
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3C
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Expression was asymmetric in the left side of the prospective floor plate.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Note:
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4 somite stage. Transverse section of the embryo in Fig. 3B.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural plate |
Present |
Regionally restricted |
3D
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Expression was detected in both sides of the prospective floor plate.
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| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3D
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Expression was detected in both sides of the lateral plate mesoderm.
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