Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:174145
Ningxia Z, et al., The expression pattern of polycomb group protein ezh2 during mouse embryogenesis. Anat Rec (Hoboken). 2011 Jul;294(7):1150-7
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:5141081
|
Gene symbol:
|
Ezh2
|
Gene name:
|
enhancer of zeste 2 polycomb repressive complex 2 subunit
|
Antibody:
|
anti-Ezh2 (AC22; #3147)
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Results
|
Specimen
Not shown E6.5:
embryonic day 6.5
(more )
Specimen
Not shown E6.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 6.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo |
Moderate |
|
|
|
Specimen
1A:
embryonic day 7.5
(more )
Specimen
1A:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Not shown E8.5:
embryonic day 8.5
(more )
Specimen
Not shown E8.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
1B:
embryonic day 9.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2A:
embryonic day 10.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: nervous system |
Strong |
|
2A
|
Expression is in almost all the nerve cells and neuroglial cells, absent in nerve fibers.
|
Specimen
2B:
embryonic day 11.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Not shown E11.5:
embryonic day 11.5
(more )
Specimen
Not shown E11.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2C:
embryonic day 17.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: nervous system |
Absent |
|
2C
|
Absent in neurons and neuroglial cells.
|
Specimen
3A:
embryonic day 15.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3B:
embryonic day 12.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: liver |
Moderate |
|
3B
|
Expression is in most liver and Kupffer cells.
|
Specimen
3C:
embryonic day 17.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: liver |
Trace |
|
3C
|
|
Specimen
3D:
embryonic day 15.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Strong |
|
3D
|
Expression is in both acinar and tubular epithelial cells, absent in other parts of the pancreas.
|
Specimen
4A:
embryonic day 14.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: renal tubule |
Present |
Regionally restricted |
4A
|
Expression is in the nuclei of renal tubule epithelial cells.
|
Specimen
4B:
embryonic day 18.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: renal tubule |
Weak |
Regionally restricted |
4B
|
Expression is in the nuclei of renal tubule epithelial cells.
|
Specimen
5A:
embryonic day 12.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: lung |
Strong |
|
5A
|
|
Specimen
5B:
embryonic day 13.5
(more )
Specimen
5B:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: lobar bronchus |
Strong |
Regionally restricted |
5B
|
Expression is in parts of pulmonary alveoli and some bronchial branches.
|
Specimen
5C:
embryonic day 18.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lung |
Absent |
|
5C
|
|
Specimen
6A:
embryonic day 10.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
6B:
embryonic day 17.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: heart |
Absent |
|
6B
|
Ambiguous: the text and figure indicate expression is absent but the table states that there was weak expression.
|
Specimen
Table 2 E10.5:
embryonic day 10.5
(more )
Specimen
Table 2 E10.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 2 E11.5:
embryonic day 11.5
(more )
Specimen
Table 2 E11.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 2 E12.5:
embryonic day 12.5
(more )
Specimen
Table 2 E12.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 2 E13.5:
embryonic day 13.5
(more )
Specimen
Table 2 E13.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 2 E14.5:
embryonic day 14.5
(more )
Specimen
Table 2 E14.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 2 E15.5:
embryonic day 15.5
(more )
Specimen
Table 2 E15.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 2 E16.5:
embryonic day 16.5
(more )
Specimen
Table 2 E16.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 2 E17.5:
embryonic day 17.5
(more )
Specimen
Table 2 E17.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Table 2 E18.5:
embryonic day 18.5
(more )
Specimen
Table 2 E18.5:
(close )
Genetic Background:
|
Kunming
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: heart |
Trace |
|
|
|
TS26: intestine mucosa |
Trace |
|
|
|
TS26: liver |
Trace |
|
|
|
TS26: nervous system |
Absent |
|
|
Absent to trace in nerve cells and absent in nerve fiber. Ambiguous: the text indicates it is absent but the table shows a that it was either absent to a trace amount in nerve cells.
|
TS26: lung |
Absent |
|
|
Ambiguous: the text and figure indicate expression is absent but the table states that there was absent to trace amounts of expression.
|
TS26: glomerular tuft |
Absent |
|
|
|
|