Antibody:	ERG MAb
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: blood vessel	Strong	Regionally restricted	4A	Strong expression in endothelial cells of blood vessels around the neural tube.
TS15: amnion	Strong	Regionally restricted	4A	Expression also prominent in the cells that line the amnion.
TS15: future hindbrain	Absent		4A
TS15: neural tube	Absent		4A
TS15: optic vesicle	Absent		4A

Structure	Level	Pattern	Image	Note
TS15: blood vessel	Strong	Regionally restricted	4B	Strong expression in endothelial cells of blood vessels around the neural tube.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle endocardial lining	Present	Regionally restricted	4C	Expression in endothelial cells that line the trabeculated regions of the ventricle.

Structure	Level	Pattern	Image	Note
TS15: blood vessel	Strong	Regionally restricted	4D	Strong expression in endothelial cells in inter-somitic capillaries

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present	Regionally restricted	4E	Endothelial-specific expression in the dorsal aorta.
TS15: tail	Present	Regionally restricted	4E	Endothelial-specific expression around the neural tube.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	5A	Endothelial cell-specific expression.
TS20: nasal septum mesenchyme	Present	Regionally restricted	5A	Specific expression in the cartilage primordium of the nasal septum and midshaft region of the rib.
TS20: rib pre-cartilage condensation	Present	Regionally restricted	5A	Specific expression in the cartilage primordium of the nasal septum and midshaft region of the rib.

Structure	Level	Pattern	Image	Note
TS20: ventricle endocardial lining	Strong	Regionally restricted	5B	Strong expression in the endothelial cells along the trabeculated endocardium.

Structure	Level	Pattern	Image	Note
TS20: vertebral pre-cartilage condensation	Present	Regionally restricted	5C	Expression in the precartilage condensations in the neural arch.

Structure	Level	Pattern	Image	Note
TS20: lung vascular element	Strong	Regionally restricted	5D	Strong expression in capillaries in the stroma.
TS20: lobar bronchus	Absent		5D	No expression in the epithelial cells of segmental bronchus.

Structure	Level	Pattern	Image	Note
TS20: developing vasculature of metanephros	Present	Uniform	5E	Uniform expression in endothelial cells of blood vessels and capillaries throughout the kidney.
TS20: metanephros	Present	Regionally restricted	5E	Authors report that expression was not detected in the kidney cortex or medulla.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Regionally restricted	6A	Expression in endothelial cells.
TS22: nasal septum	Present	Regionally restricted	6A	Specific expression in cartilage primordium (cp) of the nasal septum.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	6B	Expression in capillaries.

Structure	Level	Pattern	Image	Note
TS22: ventricle endocardial lining	Strong	Regionally restricted	6C	Strong expresssion in endothelial cells (ec) along the trabeculated endocardium.

Structure	Level	Pattern	Image	Note
TS22: lung vascular element	Strong	Regionally restricted	6D
TS22: lobar bronchus epithelium	Absent		6D

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Present	Regionally restricted	6E	Specific expression in endothelial cells.

Structure	Level	Pattern	Image	Note
TS25: ventricle endocardial lining	Present	Regionally restricted	7A

Structure	Level	Pattern	Image	Note
TS25: lung vascular element	Present	Regionally restricted	7B	Specific expression in capillaries and blood vessels.`
TS25: lung epithelium	Absent		7B
TS25: lobar bronchus	Present	Regionally restricted	7B	No expression in bronchial epithelia.

Structure	Level	Pattern	Image	Note
TS25: liver	Present	Regionally restricted	7C	Specific expression in non-hepatocytes in addition to endothelial cells.

Structure	Level	Pattern	Image	Note
TS25: adrenal cortex	Present	Regionally restricted	7D	Random expression in both cortex and medullary regions consisted with fenestrated vessels.
TS25: adrenal medulla	Present	Regionally restricted	7D	Random expression in both cortex and medullary regions consisted with fenestrated vessels.

Structure	Level	Pattern	Image	Note
TS25: metanephros	Present	Regionally restricted	7E	Specific expression in endothelial cells.
TS25: renal tubule	Absent		7E	No expression in kidney tubules.

Structure	Level	Pattern	Image	Note
TS25: intestine	Present	Regionally restricted	7F	Specific expression in endothelial cells.
TS25: intestine epithelium	Absent		7F	No expression in intestinal epithelial cells in the crypts.

Structure	Level	Pattern	Image	Note
TS25: nasal septum mesenchyme	Weak	Regionally restricted		Expression in cartilage of the rib, nasal septum and vertebra, significantly reduced compared to E12.5.
TS25: rib	Weak	Regionally restricted		Expression in cartilage of the rib, nasal septum and vertebra, significantly reduced compared to E12.5.
TS25: vertebra	Weak	Regionally restricted		Expression in cartilage of the rib, nasal septum and vertebra, significantly reduced compared to E12.5.

Structure	Level	Pattern	Image	Note
TS25: prostate bud	Present	Regionally restricted		Expression restricted to capillary endothelial cells.

Structure	Level	Pattern	Image	Note
TS25: liver	Present	Regionally restricted	8A	Expression in endothelial and other non-parenchymal cells. No expression in megakaryocytes.

Structure	Level	Pattern	Image	Note
TS28: blood vessel endothelium	Present

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present	Regionally restricted	9A	Expression in endothelial cells. No expression in megakaryocytes.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Regionally restricted	9B	Expression in endothelial cells. Expression appears to be in the Kupffer cells.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present	Regionally restricted	9C	Expression in endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: pancreas	Present	Regionally restricted	9D	Expression in endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present	Regionally restricted	9E	Expression in lymphatic endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: spleen	Present	Regionally restricted	9F	Expression in endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Present	Regionally restricted	9G	Expression in endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: thymus	Present	Regionally restricted	9H	Expression in endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: anterior prostate gland	Present	Regionally restricted	9I	Expression in endothelial cells.
TS28: prostate epithelium	Absent		9I

Structure	Level	Pattern	Image	Note
TS28: prostate gland dorsolateral lobe	Present	Regionally restricted	9J	Expression in endothelial cells of the dorsal lobe.
TS28: prostate epithelium	Absent		9J

Structure	Level	Pattern	Image	Note
TS28: prostate gland dorsolateral lobe	Present	Regionally restricted	9K	Expression in endothelial cells of the lateral lobe.
TS28: prostate epithelium	Absent		9K

Structure	Level	Pattern	Image	Note
TS28: ventral prostate gland	Present	Regionally restricted	9L	Expression in endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: adrenal gland	Present	Regionally restricted		Expression in the endothelial cells of the adrenal gland, cartilaginous component of bone, heart muscle, urinary bladder.
TS28: bladder	Present	Regionally restricted		Expression in the endothelial cells of the adrenal gland, cartilaginous component of bone, heart muscle, urinary bladder.
TS28: cardiac muscle tissue	Present	Regionally restricted		Expression in the endothelial cells of the adrenal gland, cartilaginous component of bone, heart muscle, urinary bladder.
TS28: cartilage	Present	Regionally restricted		Expression in the endothelial cells of the adrenal gland, cartilaginous component of bone, heart muscle, urinary bladder.