Reporter:	lacZ
	Direct Detection

Structure	Cell Type	Level	Pattern	Image	Note
TS15: embryo	neural crest cell	Present	Patchy	2A	Staining is observed in the expected distribution pattern of neural crest cells.
TS15: cranial preganglion	mesenchymal cell	Present	Patchy	2A	Staining is observed in the cranial sensory ganglia primordia.
TS15: fronto-nasal process		Present	Patchy	2A	Staining is observed in the frontonasal region.
TS15: branchial arch mesenchyme derived from neural crest	mesenchymal cell	Present	Patchy	2A	Staining is observed in the ventral craniofacial mesenchyme in the pharyngeal arches.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: embryo	neural crest cell	Strong		2B	Staining is observed in the expected distribution pattern of neural crest cells including the trigeminal nerve.
TS17: head mesenchyme derived from neural crest	neural crest cell	Strong		2B	Staining is observed in neural crest cells from the midbrain contributing to the periocular skeleton and connective tissues of the ventral face.
TS17: trigeminal ganglion		Strong		2B	Staining is observed in neural crest cells from the midbrain and hindbrain that will populate the trigeminal ganglia.
TS17: 1st branchial arch mandibular component	neural crest cell	Strong		2B	Staining is observed in neural crest cells from the hindbrain that populate the mandibular and hyoid arches.
TS17: 2nd branchial arch mesenchyme derived from neural crest	neural crest cell	Strong		2B	Staining is observed in neural crest cells from the hindbrain that populate the mandibular and hyoid arches.
TS17: 3rd branchial arch mesenchyme derived from neural crest	neural crest cell	Strong			Staining is observed in neural crest cells from the hindbrain that migrate to the 3rd and more caudal branchial arches and peripheral ganglia.
TS17: 4th branchial arch mesenchyme derived from neural crest	neural crest cell	Strong			Staining is observed in neural crest cells from the hindbrain that migrate to the 3rd and more caudal branchial arches and peripheral ganglia.
TS17: trunk mesenchyme derived from neural crest		Strong			Staining is observed as a confluent cell mass between the neural tube and dermomyotome at the level of the anterior half of the somite.

Structure	Level	Pattern	Image	Note
TS20: brachial plexus	Present		2C	Staining is observed in peripheral nervous system components, such as the brachial plexus and the lumbar plexus.
TS20: dorsal root ganglion	Present		2C	Staining is observed in peripheral nervous system components, such as the brachial plexus and the lumbar plexus.
TS20: peripheral nerve trunk	Present		2C	Staining is observed in peripheral nervous system components, such as the brachial plexus and the lumbar plexus.

Structure	Level	Pattern	Image	Note
TS20: brachial plexus	Present		3A
TS20: cranial nerve	Present		3A	Staining is observed in the vagal nerve.
TS20: dorsal root ganglion	Present		3A	Staining is observed in the sympathetic trunk (sympathetic nervous system), enteric nervous system and spinal dorsal root ganglia.
TS20: enteric nervous system	Present		3A	Staining is observed in the sympathetic trunk (sympathetic nervous system), enteric nervous system and spinal dorsal root ganglia.
TS20: peripheral nerve trunk	Present		3A	Staining is observed in the sympathetic trunk (sympathetic nervous system), enteric nervous system and spinal dorsal root ganglia.
TS20: sympathetic nervous system	Present		3A	Staining is observed in the sympathetic trunk (sympathetic nervous system), enteric nervous system and spinal dorsal root ganglia.
TS20: epidermis	Present		3A	Staining is observed in melanocytes in the skin.

Structure	Cell Type	Level	Pattern	Image	Note
TS15: branchial arch mesenchyme derived from neural crest	mesenchymal cell	Present		3C	Staining is observed in the mesenchyme of pharyngeal arches 2, 3, and 4.

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present		3D	Staining is observed in the enteric nervous system, with staining along the axonal distribution (supporting cells or the axons are staining in the ENS).

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		3F , 3E	Staining is observed in the spinal dorsal root ganglia, primarily in the cell bodies of the sensory neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: head mesenchyme	mesenchymal cell	Present		4A	Staining is observed in mesenchymal cells of the whole area of the pharyngeal arches and the frontonasal region, including the mesenchymal sheath covering the telencephalon. Posterior mesenchyme (forming occipital cartilage, vertebrae) is weakly stained.
TS20: head		Present		4A	Staining is observed in the alisphenoid and basisphenoid primordia.
TS20: cranium	mesenchymal cell	Present	Regionally restricted	4A	Staining is observed in mesenchymal cells of the skull base primordium rostral to the hypophyseal foramen.
TS20: facial ganglion		Present		4A
TS20: glossopharyngeal ganglion		Present		4A
TS20: trigeminal ganglion		Present		4A
TS20: vagus ganglion		Present		4A
TS20: vestibulo-cochlear ganglion		Present		4A
TS20: central nervous system ganglion		Present		4A	Staining is observed in the proximal ganglia of trigeminal (V), facial (VII), acoustic (VIII), glossopharyngeal (IX) and vagus (X) cranial nerves.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: central nervous system ganglion		Present		4B
TS20: glossopharyngeal ganglion		Present		4B
TS20: trigeminal ganglion		Present		4B
TS20: vagus ganglion		Present		4B
TS20: vestibulo-cochlear ganglion		Present		4B
TS20: facial ganglion		Present		4B	Staining is observed in the proximal ganglia of trigeminal (V), facial (VII), acoustic (VIII), glossopharyngeal (IX) and vagus (X) cranial nerves.
TS20: head mesenchyme	mesenchymal cell	Present		4B	Staining is observed in mesenchymal cells of the whole area of the pharyngeal arches and the frontonasal region, including the mesenchymal sheath covering the telencephalon. Posterior mesenchyme (forming occipital cartilage, vertebrae) is weakly stained.
TS20: cranium	mesenchymal cell	Present		4B	Staining is observed in mesenchymal cells of the skull base primordium rostral to the hypophyseal foramen.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: outflow tract	mesenchymal cell	Strong		4C	Staining is observed in the mesenchyme of the outflow tract.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: outflow tract	mesenchymal cell	Strong		4D	Staining is observed in the mesenchyme of the outflow tract.
TS20: arch of aorta	mesenchymal cell	Present		4D	Staining is observed in cells in the wall of the aortic arch artery. These are contiguous with the positive outflow tract mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: enteric nervous system	Present		5D , 5C	Staining is observed in neuronal precursors (enteric ganglia) of the enteric nervous system, in cells that also label for HoxB5
TS21: foregut	Present		5D , 5C	Staining is observed in neuronal precursors (enteric ganglia) of the enteric nervous system, in cells that also label for HoxB5

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		5F , 5E	Staining is observed in subsets of cells of spinal dorsal root ganglia. These cells are also positive for Islet-1.
TS21: trigeminal ganglion	Present		5E , 5F	Staining is observed in subsets of cells of the trigeminal ganglia. These cells are also positive for Islet-1.

Structure	Level	Pattern	Image	Note
TS21: sympathetic ganglion	Present			Staining is observed in subsets of cells of sympathetic ganglia. These cells are also positive for Islet-1.