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Gene Expression Data
Assay Details
Assay
Reference: J:139151 Bullard T, et al., Ascl3 expression marks a progenitor population of both acinar and ductal cells in mouse salivary glands. Dev Biol. 2008 Aug 1;320(1):72-8
Assay type: Recombinase reporter
MGI Accession ID: MGI:5014324
Gene symbol: Ascl3
Gene name: achaete-scute family bHLH transcription factor 3
Reporter: lacZ
Direct Detection
Results
Specimen Fig. 3A: embryonic day 13.5; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: salivary gland Absent 3A No staining is observed, indicating no Ascl1-driven cre expression at the early bud or pseudoglandular stage.
Specimen Fig. 3B: embryonic day 15.5; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: salivary gland Present Regionally restricted 3B Staining is observed in cells in the large excretory duct in the salivary gland at the canalicular stage.
Specimen Fig. 3C: embryonic day 17.5; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: salivary gland Present Regionally restricted 3C Staining is observed in cells in ductal structures at the terminal bud stage. No staining is observed in the terminal end buds (presumptive acinar cells.)
Specimen not shown: embryonic day 17.5; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: sublingual gland Present Regionally restricted Staining is observed in cells in ductal structures at the terminal bud stage in all three glands (submandibular, sublingual and parotid). No staining is observed in the terminal end buds (presumptive acinar cells.)
TS25: submandibular gland Present Regionally restricted Staining is observed in cells in ductal structures at the terminal bud stage in all three glands (submandibular, sublingual and parotid). No staining is observed in the terminal end buds (presumptive acinar cells.)
Specimen Fig. 4A: postnatal week 3; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: submandibular gland Present 4A Ectopic staining is observed in acinar cells, derived from by lacZ-stained progeny of Ascl1-cre expressing cells.
TS28: submandibular duct Present 4A Staining is observed in duct cells.
Specimen Fig. 4B: postnatal week 3; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: sublingual gland Present 4B Staining is observed in duct cells as expected, and (ectopically) in a signficant number of acinar cells.
Specimen Fig. 4C: postnatal week 3; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: parotid gland Present 4C Staining is observed in duct cells as expected, and (ectopically) in a signficant number of acinar cells.
Specimen Fig. 5A: postnatal week 6; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: submandibular gland Present 5A Staining is observed in some cells in the apical membranes of acinar cells which also express aquaporin 5.
TS28: submandibular duct Present 5A Staining is observed in duct cells which do not express aquaporin 5.
Specimen Fig. 5B: postnatal week 6; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: submandibular gland Present 5B Staining is observed in cells in the acinar compartment which do not label for cytokeratin 19 (duct cell marker).
TS28: submandibular duct Present 5B Staining is observed in duct cells which co-label for cytokeratin 19 (duct cell marker).
Specimen Fig. 6A: postnatal week 3; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: sublingual gland Present Single cells 6A Staining is observed in duct cells.
Specimen Fig. 6B: postnatal month 3; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: sublingual gland Present Scattered 6B Staining is observed in duct cells with an increasing contribution to the acinar cell compartment (including serous demilune cells) by lacZ-stained progeny of Ascl1-cre expressing cells (with age).
Specimen Fig. 6C: postnatal year 1; Ascl3tm1(EGFP/cre)Ovi/Ascl3+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: sublingual gland Present Widespread 6C Staining is observed in duct cells with a large contribution to the acinar cell compartment (including serous demilune cells) by lacZ-stained progeny of Ascl1-cre expressing cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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