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Gene Expression Data
Assay Details
Assay
Reference: J:147577 Takahashi N, et al., Deletion of Gtl2, imprinted non-coding RNA, with its differentially methylated region induces lethal parent-origin-dependent defects in mice. Hum Mol Genet. 2009 May 15;18(10):1879-88
Assay type: RT-PCR
MGI Accession ID: MGI:5014268
Gene symbol: Dlk1
Gene name: delta like non-canonical Notch ligand 1
Probe: Dlk1-pG, Dlk1-pH
Probe preparation: labelled with SYBR green
Assay notes: Quantitative real-time RT-PCR assay. Graphical representation of the expression level relative to that of GAPDH expression.
Results Image: 3A Dlk1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
B P1 TS27: brain Present 0.5 µg; total RNA Not Specified Not Specified
B P1 TS27: brain Present (a) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
B P1 TS27: brain Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
B P1 TS27: brain Present (c) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
T P1 TS27: tongue Present 0.5 µg; total RNA Not Specified Not Specified
T P1 TS27: tongue Present (a) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
T P1 TS27: tongue Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
T P1 TS27: tongue Present (c) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
Lu P1 TS27: lung Present 0.5 µg; total RNA Not Specified Not Specified
Lu P1 TS27: lung Present (a) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
Lu P1 TS27: lung Present (d) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
Lu P1 TS27: lung Present (e) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
Li P1 TS27: liver Present 0.5 µg; total RNA Not Specified Not Specified
Li P1 TS27: liver Present (a) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
Li P1 TS27: liver Present (d) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
Li P1 TS27: liver Present (f) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
Notes:
(a) There was no significant change in the expression of paternally expressed Dlk1 in the Maternal-KO mutant compared to wild-type.
(b) Expression was decreased in the Paternal-KO mutant compared to wild-type.
(c) Expression was decreased compared to wild-type.
(d) Expression was decreased to <15% of wild-type levels.
(e) Expression was decreased to 23% of the control level.
(f) Expression was decreased to 58% of the control level.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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