Hs3st4 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:103005 Yabe T, et al., Developmental and regional expression of heparan sulfate sulfotransferase genes in the mouse brain. Glycobiology. 2005 Oct;15(10):982-93
Assay type:	RNA in situ
MGI Accession ID:	MGI:4949711
Gene symbol:	Hs3st4
Gene name:	heparan sulfate (glucosamine) 3-O-sulfotransferase 4

Probe:	3-OST-4 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 2C & 2H: embryonic day 16.0 (more

)
Note: Fig. 2H is a higher magnification of 2C.

Structure	Level	Image
TS24: cerebral cortex ventricular layer	Present	2H , 2C
TS24: cortical plate	Present	2H , 2C
TS24: cerebral cortex intermediate zone	Weak	2C , 2H
TS24: cerebral cortex marginal layer	Absent	2C , 2H
TS24: cortical subplate	Absent	2C , 2H

Specimen 5D: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Weak	Regionally restricted	5D	Signal was detected in the pyramidal neurons in layer V.

Specimen not shown: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Strong
TS27: cerebellum external granule cell layer	Strong

Specimen not shown: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Weak

Specimen not shown: postnatal day 14 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present
TS28: cerebellum internal granule cell layer	Present

Specimen not shown: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present
TS28: cerebellum granule cell layer	Present
TS28: cerebellum molecular layer	Weak

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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