Probe:	3-OST-2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Present		2B
TS24: cortical plate	Present		2B
TS24: cerebral cortex intermediate zone	Weak		2B

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		2F

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	3B	Expression was detected in cells in the marginal zone. Positive signals were detected in radially migrating cells in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		3F
TS28: cortical layer III	Present		3F
TS28: cortical layer IV	Strong	Regionally restricted	3F	Expression was strong in a subset of neurons.
TS28: cortical layer V	Weak		3F
TS28: cortical layer VI	Weak		3F

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present		3J	Significant signal was observed in all the layers.
TS28: cortical layer II	Strong	Single cells	3J	Expression was strong in a few neurons.
TS28: cortical layer III	Strong	Single cells	3J	Expression was strong in a few neurons.
TS28: cortical layer V	Strong	Single cells	3J	Expression was strong in a few neurons.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		4A	Expression signal was intense in most neurons in layers II/III. A subset of neurons in layer IV also expressed the transcript intensely.
TS28: cortical layer III	Present		4A	Expression signal was intense in most neurons in layers II/III. A subset of neurons in layer IV also expressed the transcript intensely.
TS28: cortical layer IV	Present		4A	Expression signal was intense in most neurons in layers II/III. A subset of neurons in layer IV also expressed the transcript intensely.

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Strong	Regionally restricted	5A , 5E	Strong signals were detected in the pyramidal neurons in layer V.
TS28: cortical layer II	Strong		5A , 5E
TS28: cortical layer III	Strong		5A , 5E

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		7A
TS27: cerebellum external granule cell layer	Present		7A

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		7C
TS28: cerebellum internal granule cell layer	Present		7C
TS28: cerebellum Purkinje cell layer	Present	Single cells	7C	Expression was detected in some of the cells in the molecular layer and a few Purkinje cells.
TS28: cerebellum molecular layer	Present	Single cells	7C	Expression was detected in some of the cells in the molecular layer and a few Purkinje cells.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		8A
TS28: cerebellum internal granule cell layer	Strong		8A
TS28: cerebellum external granule cell layer	Present		8A
TS28: cerebellum molecular layer	Present	Single cells	8A	A few positive interneurons were also observed in the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		8C
TS28: cerebellum granule cell layer	Strong		8C

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present		8E	Expression was detected in a number of interneurons in the molecular layer.