Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:135242
Kala K, et al., Analysis of the midbrain-hindbrain boundary cell fate using a boundary cell-specific Cre-mouse strain. Genesis. 2008 Jan;46(1):29-36
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:4949456
|
|
Gene symbol:
|
En1
|
|
Gene name:
|
engrailed 1
|
|
Reporter:
|
Cre
|
|
Antibody:
|
anti-cre (Sigma)
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 568
|
|
|
Results
|
Specimen
1l:
embryonic day 10.5; En1tm7(cre/ESR1)Alj/En1 +, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +
(more  )
Note: Conditional mutant. Sagittal section. Double labeled: green - En1 reporter (Cre); red - Cre reporter.
Specimen
1l:
(close  )
|
Genetic Background:
|
involves: 129S4/SvJaeSor * 129S6/SvEvTac
|
|
Mutant Allele(s):
|
En1tm7(cre/ESR1)Alj/En1+
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Conditional mutant. Sagittal section. Double labeled: green - En1 reporter (Cre); red - Cre reporter.
|
|
Analysis Tools
