About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:135242 Kala K, et al., Analysis of the midbrain-hindbrain boundary cell fate using a boundary cell-specific Cre-mouse strain. Genesis. 2008 Jan;46(1):29-36
Assay type: Recombinase reporter
MGI Accession ID: MGI:4949441
Gene symbol: Tg(Hoxb7-cre)1Jpa
Gene name: transgene insertion 1, Juha Partanen
Reporter: lacZ
Antibody: anti-B-galactosidase (55976)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Secondary antibodies are labeled with AlexaFluor 488/568 or horseradish peroxidase in Fig. 5g.
Results
Specimen Fig. 1c: embryonic day 10.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Hoxb7-cre)1Jpa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: midbrain-hindbrain junction Present 1e , 1c Staining is observed in a group of cells at the midbrain-hindbrain boundary.
Specimen Fig. 1h: embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Hoxb7-cre)1Jpa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Present Regionally restricted 1h , 1j Labeling is abundant in the dorsal region close to the midbrain-hindbrain boundary.
Specimen Fig. 2a: embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Hoxb7-cre)1Jpa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Present Non-Uniform 2a Labeling is strong in dorsal boundary region and weaker in the ventral part.
TS19: hindbrain Present Regionally restricted 2a Labeling is observed in a cell population within the Cdh22 expression domain and a subset of cells overlapping with the p21 expressing cells in the most posterior midbrain and the hindbrain in the most anterior rhombomere 1 of hindbrain.
TS19: midbrain Present Regionally restricted 2a Labeling is observed in a cell population within the Cdh22 expression domain and a subset of cells overlapping with the p21 expressing cells in the most posterior midbrain and the hindbrain in the most anterior rhombomere 1 of hindbrain.
Specimen Fig. 3d: embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Hoxb7-cre)1Jpa/0 (more )
Note: Conditional mutant. Coronal section.
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Present 3d Labeling is observed in some p21-expressiing cells at the midbrain-rhombomere 1 boundary, but most beta-gal labeled cells are not p21-positive.
Specimen Fig. 5e: postnatal day 60; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Hoxb7-cre)1Jpa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present Spotted 5e Labeling is observed in Purkinje cells.
TS28: cerebellum molecular layer Present Spotted 5e Labeling is observed in Purkinje cells and stellate cells in the molecular layer.
Specimen Fig. 5f: postnatal day 60; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Hoxb7-cre)1Jpa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: cerebellum molecular layer Present Spotted 5f Staining is observed in basket cells in the molecular layer.
Specimen Fig. 5g: postnatal day 60; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Hoxb7-cre)1Jpa/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: cerebellum granule cell layer Present Spotted 5g Staomomg is observed in a few cells (Golgi interneurons) in the granule layer.
Specimen Fig. 5j: embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Hoxb7-cre)1Jpa/0 (more )
Note: Conditional mutant. Sagittal section.
Structure Level Pattern Image Note
TS26: midbrain Present 5j Labeling is observed in few cells that are near serotonergic neurons which label for 5-hydroxy tryptophan.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory