Gene Expression Data
Assay Details
Assay
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Reference:
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J:73470
Kim M, et al., Expression pattern of HSP25 in mouse preimplantation embryo: Heat shock responses during oocyte maturation. Mol Reprod Dev. 2002 Jan;61(1):3-13
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4949248
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Gene symbol:
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Hspb1
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Gene name:
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heat shock protein 1
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Antibody:
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anti-HSP25
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Embryos were fixed in 2.5% neutral buffered paraformaldehyde.
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Results
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Specimen
3A:
embryonic day 0.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
involves: C57BL * CBA
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Age:
|
embryonic day 0.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Paraformaldehyde
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Specimen
3C:
embryonic day 0.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
involves: C57BL * CBA
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Age:
|
embryonic day 0.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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Paraformaldehyde
|
Specimen
3E:
embryonic day 1.0
(more )
Specimen
3E:
(close )
Genetic Background:
|
involves: C57BL * CBA
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Age:
|
embryonic day 1.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
Paraformaldehyde
|
Specimen
3G:
embryonic day 2.0
(more )
Specimen
3G:
(close )
Genetic Background:
|
involves: C57BL * CBA
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Age:
|
embryonic day 2.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
Paraformaldehyde
|
Specimen
3I:
embryonic day 3.0
(more )
Specimen
3I:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 3.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
Paraformaldehyde
|
Specimen
3K:
embryonic day 3.5
(more )
Specimen
3K:
(close )
Genetic Background:
|
involves: C57BL * CBA
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Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Present |
|
3K
|
Expression is detected in the cytoplasm.
|
Specimen
6A:
postnatal
(more )
Specimen
6A:
(close )
Genetic Background:
|
ICR
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Age:
|
postnatal
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
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6A
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In germinal vesicle-oocytes, the protein was detected in both the cytoplasm and nucleus; it was not present in the nucleolus.
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Specimen
6E:
postnatal
(more )
Specimen
6E:
(close )
Genetic Background:
|
ICR
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Age:
|
postnatal
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
|
6E
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The protein is distributed in the whole cytoplasm in germinal vesicle breakdown-oocytes.
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Specimen
6I:
postnatal
(more )
Specimen
6I:
(close )
Genetic Background:
|
ICR
|
Age:
|
postnatal
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
|
6I
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The protein is distributed in the whole cytoplasm in metaphase II-oocytes.
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