Gene Expression Data
Assay Details
Assay
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Reference:
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J:105459
Jepeal LI, et al., Cell-specific expression of glucose-dependent-insulinotropic polypeptide is regulated by the transcription factor PDX-1. Endocrinology. 2005 Jan;146(1):383-91
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4948726
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Gene symbol:
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Gip
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Gene name:
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gastric inhibitory polypeptide
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Antibody:
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Anti-GIP
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
1C & 1D:
embryonic day 18.5
(more )
Note: Double labeled: red - Gip; blue - Pdx1 (assay MGI:4948721).
Specimen
1C & 1D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Gip; blue - Pdx1 (assay MGI:4948721).
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Specimen
1G & 1H:
postnatal week 10
(more )
Note: Double labeled: red - Gip; blue - Pdx1 (assay MGI:4948721).
Specimen
1G & 1H:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Gip; blue - Pdx1 (assay MGI:4948721).
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Specimen
2A:
embryonic day 18.5
(more )
Specimen
2A:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2B:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Pdx1tm1Cvw/Pdx1tm1Cvw
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: duodenum |
Trace |
Regionally restricted |
2B
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A graph quantifying immunopositive cells indicates that Gip-positive cells decreased 97.8% in mutant embryos compared to wild type. A few positive cells were detected in the distal portion but not in the proximal portion of the duodenum in mutant embryos.
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