Gene Expression Data
Assay Details
Assay
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Reference:
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J:104961
St Germain C, et al., Expression and transient nuclear translocation of proprotein convertase 1 (PC1) during mouse preimplantation embryonic development. Mol Reprod Dev. 2005 Dec;72(4):483-93
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4947707
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Gene symbol:
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Pcsk1
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Gene name:
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proprotein convertase subtilisin/kexin type 1
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Antibody:
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anti-PC1-CT
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Assay notes:
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The antibody used in this assay only recognizes the isoforms of the protein which contain the C-terminus.
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Results
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Specimen
3Aa:
postnatal
(more )
Note: Unfertilized egg.
Specimen
3Aa:
(close )
Genetic Background:
|
CF-1
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Age:
|
postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
|
optical section
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Fixation:
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Formaldehyde
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Note:
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Unfertilized egg.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Uniform |
3Aa
|
There is even staining in the cytoplasm.
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Specimen
3Ab:
embryonic day 0.5
(more )
Note: 1 cell embryo.
Specimen
3Ab:
(close )
Genetic Background:
|
CF-1
|
Age:
|
embryonic day 0.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
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Fixation:
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Formaldehyde
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Note:
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1 cell embryo.
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Specimen
3Ac:
embryonic day 0.5
(more )
Note: 1 cell embryo.
Specimen
3Ac:
(close )
Genetic Background:
|
CF-1
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Age:
|
embryonic day 0.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Note:
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1 cell embryo.
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Specimen
3Ad:
embryonic day 1.0
(more )
Note: 2 cell embryo.
Specimen
3Ad:
(close )
Genetic Background:
|
CF-1
|
Age:
|
embryonic day 1.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
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Type:
|
optical section
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Fixation:
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Formaldehyde
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Note:
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2 cell embryo.
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Specimen
3Ae:
embryonic day 2.5
(more )
Note: 6-8 cell embryo.
Specimen
3Ae:
(close )
Genetic Background:
|
CF-1
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Age:
|
embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
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Formaldehyde
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Note:
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6-8 cell embryo.
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Specimen
3Af:
embryonic day 3.0
(more )
Note: Morula.
Specimen
3Af:
(close )
Genetic Background:
|
CF-1
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Age:
|
embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
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Formaldehyde
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Note:
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Morula.
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
Non-Uniform |
3Af
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Expression is most intense at blastomere junctions.
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Specimen
3Ag:
embryonic day 3.5
(more )
Note: Blastocyst.
Specimen
3Ag:
(close )
Genetic Background:
|
CF-1
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Age:
|
embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
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Formaldehyde
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Note:
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Blastocyst.
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Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Present |
Non-Uniform |
3Ag
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Expression is most intense at blastomere junctions.
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Specimen
4A:
embryonic day 3.5
(more )
Specimen
4A:
(close )
Genetic Background:
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CF-1
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Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
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Type:
|
optical section
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Fixation:
|
Formaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Present |
|
4Aa
,
4Ac
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Expression is present in the cytoplasm.
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Specimen
4B:
embryonic day 3.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
CF-1
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Age:
|
embryonic day 3.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
Formaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Present |
|
4Bc
,
4Ba
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Expression is generally non-nuclear or perinuclear.
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