Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:96871
Chi H, et al., Loss of mitogen-activated protein kinase kinase kinase 4 (MEKK4) results in enhanced apoptosis and defective neural tube development. Proc Natl Acad Sci U S A. 2005 Mar 8;102(10):3846-51
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:4947669
|
|
Gene symbol:
|
Mapk8
|
|
Gene name:
|
mitogen-activated protein kinase 8
|
|
Antibody:
|
Anti-Phospho-JNK
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Assay notes:
|
The antibody used in this assay recognized the phosphorylated form of the protein.
|
|
|
Results
|
Specimen
7A Top MEKK4+/+:
embryonic day 9.5
(more  )
Specimen
7A Top MEKK4+/+:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
7A Top MEKK4-/-:
(close )
|
Genetic Background:
|
B6.129S6-Map3k4tm1Flv
|
|
Mutant Allele(s):
|
Map3k4tm1Flv/Map3k4tm1Flv
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Strong |
Regionally restricted |
7A HB Mekk4-/-
|
Expression was stronger toward the edges of the hindbrain neural folds. There was no significant difference between +/+ and -/- embryos.
|
Specimen
7A Bottom MEKK4+/+:
embryonic day 9.5
(more )
Specimen
7A Bottom MEKK4+/+:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
7A Bottom MEKK4-/-:
(close )
|
Genetic Background:
|
B6.129S6-Map3k4tm1Flv
|
|
Mutant Allele(s):
|
Map3k4tm1Flv/Map3k4tm1Flv
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Strong |
|
7A FB Mekk4-/-
|
Expression was strong in the forebrain neuroepithelium. There was no significant difference between +/+ and -/- embryos.
|
|
Analysis Tools