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Gene Expression Data
Assay Details
Assay
Reference: J:109568 Klewer SE, et al., Has2 expression in heart forming regions is independent of BMP signaling. Gene Expr Patterns. 2006 Jun;6(5):462-70
Assay type: RNA in situ
MGI Accession ID: MGI:4941908
Gene symbol: Has2
Gene name: hyaluronan synthase 2
Probe: Has2 cDNA2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Fixative was a 6:3:1 solution of ethanol:formalin:acetic acid with a post-fix of 4% paraformaldehyde.
Results
Specimen 3A,B: embryonic day 8.0 (more )
Note: (B) Higher magnification of (A).
Structure Level Pattern Image Note
TS12: cardiac muscle tissue Present 3B , 3A
TS12: branchial arch Present 3A
TS12: heart Present 3A
TS12: neural crest Absent 3A
TS12: notochord Absent 3A

Specimen 3C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: endocardial cushion Present 3C
TS17: outflow tract Present 3C
TS17: atrioventricular canal Present Regionally restricted 3C Expression is in the endocardium.
TS17: cardiac muscle tissue Absent 3C

Specimen 3E: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: atrioventricular canal Present Regionally restricted 3E Expression is in the cushion tissue.
TS19: outflow tract Present Regionally restricted 3E Expression is in the cushion tissue.
TS19: dorsal aorta Present 3E

Specimen 3F: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: outflow tract Present Regionally restricted 3F Expression is in the cushion tissue.
TS20: atrioventricular endocardial cushion Present 3F
TS20: dorsal aorta Present 3F

Specimen 4A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: atrioventricular canal Present Regionally restricted 4A Expression is in mesenchymal cells in the luminal projections.
TS21: lung Present 4A

Specimen 4B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: atrioventricular canal Present Regionally restricted 4B Expression is in mesenchymal cells in the luminal projections.

Specimen 4C: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: outflow tract Present 4C

Specimen 4D,E: embryonic day 14.5 (more )
Note: (E) Higher magnification of (D).
Structure Level Pattern Image Note
TS22: heart valve Present 4D , 4E Expression is in the forming atrioventricular valve leaflets.

Specimen 4F: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: heart valve Present 4F Expression is in the forming semilunar valve leaflets.

Specimen 4G,H: embryonic day 16.5 (more )
Note: (H) Higher magnification of (G).
Structure Level Pattern Image Note
TS24: heart valve Present Regionally restricted 4G , 4H Expression is in the atrioventricular valve leaflets.
TS24: heart Present Regionally restricted 4G , 4H Expression is in the chordae tendineae apparatus.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory