Probe:	MH621 probe 557
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramide-biotin/streptavidin amplification step was included in the in situ hybridization procedure.

Structure	Level	Pattern	Image	Note
TS23: collecting duct	Absent		euxassay_018073_12	Expression was not detected in the ureteric tubules.
TS23: ureteric tip	Absent		euxassay_018073_12
TS23: cap mesenchyme	Strong		euxassay_018073_12	Expression is present in the condensing mesenchyme.
TS23: renal vesicle	Absent		euxassay_018073_12	Expression was not detected in the renal vesicles/comma-shaped bodies.
TS23: s-shaped body	Absent		euxassay_018073_12
TS23: cortical renal tubule	Absent		euxassay_018073_12	Expression was not detected in the proximal tubules.
TS23: maturing glomerular tuft	Absent		euxassay_018073_12	Expression was not detected in the glomeruli.
TS23: renal vasculature	Absent		euxassay_018073_12	Expression was not detected in the capillaries.
TS23: renal cortical interstitium	Absent		euxassay_018073_12	Expression was not detected in the stroma.
TS23: renal capsule	Absent		euxassay_018073_12

Structure	Level	Pattern	Image	Note
TS23: cap mesenchyme	Present		5B 3	Expression is present in the condensing mesenchyme. An inset shows the staining at higher magnification.

Structure	Level	Pattern	Image	Note
TS23: neocortex	Moderate		euxassay_018073_12
TS23: hippocampus	Moderate		euxassay_018073_12
TS23: corpus striatum	Absent		euxassay_018073_12
TS23: telencephalon septum	Absent		euxassay_018073_12
TS23: thalamus	Absent		euxassay_018073_12
TS23: hypothalamus	Weak		euxassay_018073_12
TS23: pretectum	Weak		euxassay_018073_12
TS23: superior colliculus	Weak		euxassay_018073_12
TS23: inferior colliculus	Weak		euxassay_018073_12
TS23: midbrain tegmentum	Weak		euxassay_018073_12
TS23: cerebellum	Absent		euxassay_018073_12
TS23: spinal cord	Absent		euxassay_018073_12
TS23: skin	Absent		euxassay_018073_12
TS23: lung	Weak		euxassay_018073_12
TS23: metanephros	Strong		euxassay_018073_12
TS23: liver	Weak		euxassay_018073_12
TS23: thyroid gland	Absent		euxassay_018073_12
TS23: pituitary gland	Absent		euxassay_018073_12
TS23: pancreas	Absent		euxassay_018073_12
TS23: adrenal gland	Absent		euxassay_018073_12
TS23: heart	Absent		euxassay_018073_12
TS23: thymus primordium	Absent		euxassay_018073_12
TS23: vertebral axis musculature	Absent		euxassay_018073_12
TS23: axial skeleton	Strong		euxassay_018073_12