Antibody:	clone CB-955 (#C9848)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	These specimens are also entries in the GUDMAP database; the single labeled specimen is GUDMAP:8206 and the double-labeled specimens are GUDMAP:8199.

Structure	Level	Pattern	Image	Note
TS28: cortical collecting duct	Strong		1e , 1_Calb1 low mag
TS28: renal connecting tubule	Strong		1_Calb1 low mag
TS28: distal convoluted tubule	Strong		1_Calb1 low mag
TS28: outer medullary collecting duct	Moderate	Regionally restricted	1f , 1_Calb1 low mag , 1g	There is moderate to weak expression. It is present in a subset.
TS28: inner medullary collecting duct	Moderate	Regionally restricted	1_Calb1 low mag , 1h	There is moderate to weak expression. It is present in a subset.

Structure	Level	Pattern	Image	Note
TS28: medullary collecting duct	Absent

Structure	Level	Pattern	Image	Note
TS23: urothelium of ureter	Moderate	Non-Uniform	4a	There is moderate to weak expression.
TS23: ureteric tip	Strong		4a , 4b
TS23: cortical collecting duct	Strong		4a , 4b
TS23: cortical early distal tubule	Present		4a , 4b
TS23: renal connecting tubule	Absent		4b , 4a
TS23: medullary collecting duct	Moderate		4c , 4a
TS23: pelvic urothelial lining	Moderate	Non-Uniform	4c , 4a	There is moderate to weak expression.
TS23: immature loop of Henle	Absent		4a , 4c

Structure	Level	Pattern	Image	Note
TS23: ureteric tip	Strong		4d
TS23: ureteric tree terminal branch excluding tip itself	Strong		4d	Expression in present in the ureteric tree terminal branch.
TS23: cortical collecting duct	Present		4d

Structure	Level	Pattern	Image	Note
TS23: ureteric tip	Strong		4e
TS23: renal connecting tubule	Absent		4e	There is no expression in the renal connecting tubule of the renal vesicle.

Structure	Level	Pattern	Image	Note
TS23: ureteric tip	Strong		4f

Structure	Level	Pattern	Image	Note
TS23: ureteric tip	Strong		4g
TS23: renal connecting tubule	Absent		4g

Structure	Level	Pattern	Image	Note
TS23: ureteric tip	Strong		4h
TS23: renal connecting tubule	Moderate		4h
TS23: cortical early distal tubule	Present	Non-Uniform	4h	In the early distal tubule there is weak expression and strong expression in single cells of the same nephron.
TS23: cortical collecting duct	Present		4h

Structure	Level	Pattern	Image	Note
TS23: ureteric tip	Strong		4i
TS23: renal connecting tubule	Moderate		4i
TS23: cortical early distal tubule	Present	Non-Uniform	4i	In the early distal tubule there is weak expression and strong expression in single cells of the same nephron.
TS23: cortical collecting duct	Present		4i

Structure	Level	Pattern	Image	Note
TS23: ureteric tip	Strong		4j
TS23: renal connecting tubule	Absent		4j	There is no expression in the renal connecting tubule of early nephrons.
TS23: s-shaped body	Absent		4j

Structure	Level	Pattern	Image	Note
TS23: cortical early distal tubule	Present	Single cells	4l , 4m , 4k	Expression is present in a subset. It is likely to be turned on in stage IV nephrons.

Structure	Level	Pattern	Image	Note
TS23: cortical early distal tubule	Present	Single cells	4n	Expression is present in a subset. It is likely to be turned on in stage IV nephrons.

Structure	Level	Pattern	Image	Note
TS23: metanephros	Present		6A

Structure	Level	Pattern	Image	Note
TS23: cortical collecting duct	Present		6B
TS23: ureteric tip	Present		6B
TS23: ureteric tree terminal branch excluding tip itself	Present		6B	Expression is present in the ureteric tree terminal branch.
TS23: renal connecting tubule	Absent		6B

Structure	Level	Pattern	Image	Note
TS23: medullary collecting duct	Present		6C
TS23: cortical collecting duct	Present		6C
TS23: renal connecting tubule	Present		6C
TS23: cortical early distal tubule	Present		6C