Mmp20 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:165465 Sehic A, et al., Gene expression and dental enamel structure in developing mouse incisor. Eur J Oral Sci. 2010 Apr;118(2):118-30
Assay type:	RT-PCR
MGI Accession ID:	MGI:4849221
Gene symbol:	Mmp20
Gene name:	matrix metallopeptidase 20 (enamelysin)

Probe:	Mmp20-pA, Mmp20-pB
Assay notes:	Maxillary incisor tooth germs were removed and cut into incisal tip segment and adjacent segments prior to RNA extraction.

Results

Image: 3_Mmp20

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
E17.5 tip	E17.5	TS25: upper jaw incisor	Present	Not Specified; total RNA	CD-1		Not Specified	incisor tip segment.
E17.5	E17.5	TS25: upper jaw incisor	Present	Not Specified; total RNA	CD-1		Not Specified	part of the tooth that is adjacent to the incisal tip.
P0 tip	P0	TS27: upper jaw incisor	Present (a)	Not Specified; total RNA	CD-1		Not Specified	incisor tip segment.
P0	P0	TS27: upper jaw incisor	Present (b)	Not Specified; total RNA	CD-1		Not Specified	part of the tooth that is adjacent to the incisal tip.
P1 tip	P1	TS27: upper jaw incisor	Present	Not Specified; total RNA	CD-1		Not Specified	incisor tip segment.
P1	P1	TS27: upper jaw incisor	Present (c)	Not Specified; total RNA	CD-1		Not Specified	part of the tooth that is adjacent to the incisal tip.
P2 tip	P2	TS27: upper jaw incisor	Present	Not Specified; total RNA	CD-1		Not Specified	incisor tip segment.
P2	P2	TS27: upper jaw incisor	Present (c)	Not Specified; total RNA	CD-1		Not Specified	part of the tooth that is adjacent to the incisal tip.
P10 tip	P10	TS28: upper jaw incisor	Present (d)	Not Specified; total RNA	CD-1		Not Specified	incisor tip segment.
P10	P10	TS28: upper jaw incisor	Present (d)	Not Specified; total RNA	CD-1		Not Specified	part of the tooth that is adjacent to the incisal tip.

Notes:
(a) Expression was significantly increased at P0 compared with E17.5.
(b) Expression was significantly increased at P0 compared with E17.5. Expression was found to be significantly higher in the adjacent segment than in the incisal tip segment at this age.
(c) Expression was found to be significantly higher in the adjacent segment than in the incisal tip segment at this age.
(d) Expression decreased at later developmental stages.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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