Gene Expression Data
Assay Details
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Assay
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Reference:
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J:156017
Yokoyama S, et al., A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. Dev Cell. 2009 Dec;17(6):836-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4848516
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Gene symbol:
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Smad3
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Gene name:
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SMAD family member 3
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Probe:
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Smad3 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
Table S4 - E9.5:
embryonic day 9.5
(more  )
Specimen
Table S4 - E9.5:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Strong |
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Staining is present in the metencephalon.
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| TS15: neural tube |
Strong |
Regionally restricted |
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Staining is present in the medial neural tube.
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Specimen
Table S4 - E10.5:
embryonic day 10.5
(more )
Specimen
Table S4 - E10.5:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb mesenchyme |
Strong |
Non-Uniform |
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Staining is strong in the progress zone and moderate in the middle and proximal limb.
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| TS17: limb |
Strong |
Regionally restricted |
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Staining is present in the posterior limb.
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| TS17: genitourinary system |
Moderate |
Regionally restricted |
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Staining is present in the external genitalia.
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| TS17: nasal pit |
Moderate |
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Staining is present in the olfactory placode.
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| TS17: hindbrain |
Strong |
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Staining is present in the metencephalon.
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| TS17: neural tube |
Strong |
Regionally restricted |
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Staining is present in the medial neural tube.
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Specimen
Table S4 - E11.5:
embryonic day 11.5
(more )
Specimen
Table S4 - E11.5:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb mesenchyme |
Strong |
Regionally restricted |
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Staining is present in the progress zone and middle limb.
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| TS19: limb |
Strong |
Regionally restricted |
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Staining is present in the antero-distal, posterior-distal and interdigital regions of the limb.
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| TS19: trunk somite |
Moderate |
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| TS19: genital tubercle |
Strong |
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Staining is present in the external genitalia.
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| TS19: hindbrain |
Moderate |
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Staining is present in the metencephalon.
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| TS19: neural tube |
Moderate |
Regionally restricted |
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Staining is present in the medial neural tube.
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Analysis Tools