Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		2A
TS15: 2nd branchial arch	Present		2A
TS15: primitive ventricle	Present		2A
TS15: forelimb bud	Present		2A
TS15: dorsal root ganglion	Present	Scattered	2A	Staining is observed in a few scattered cells.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component mesenchyme	Present		2A'	Staining is observed in the arch mesenchyme similar to the Hand2 expression pattern.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		2D
TS17: 2nd branchial arch	Present		2D
TS17: 3rd branchial arch	Present		2D
TS17: limb	Present		2D
TS17: primitive ventricle	Present		2D
TS17: heart atrium	Present	Scattered	2D
TS17: dorsal root ganglion	Present	Regionally restricted	2D	Staining is observed in the cervical dorsal root ganglion.

Structure	Level	Pattern	Image	Note
TS17: 4th branchial arch	Present

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Present	Regionally restricted	2D'	Staining is observed in the periphery of the arch mesenchyme.
TS17: 2nd branchial arch mesenchyme	Present	Regionally restricted	2D'	Staining is observed in the periphery of the arch mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: Meckel's cartilage	Present		3A	Staining is observed in structures derived from the first pharyngeal arch, including the mandibular bone, tongue, Meckel's cartilage and surrounding mesenchyme/connective tissue.
TS21: mandible	Present		3A	Staining is observed in structures derived from the first pharyngeal arch, including the mandibular bone, tongue, Meckel's cartilage and surrounding mesenchyme/connective tissue.
TS21: mandible mesenchyme	Present		3A	Staining is observed in structures derived from the first pharyngeal arch, including the mandibular bone, tongue, Meckel's cartilage and surrounding mesenchyme/connective tissue.
TS21: tongue	Present		3A	Staining is observed in structures derived from the first pharyngeal arch, including the mandibular bone, tongue, Meckel's cartilage and surrounding mesenchyme/connective tissue.
TS21: submandibular gland primordium mesenchyme	Present	Widespread	3A
TS21: submandibular gland primordium epithelium	Absent		3A'

Structure	Level	Pattern	Image	Note
TS26: mandible	Present	Widespread	3D	Staining is observed in cells along the entire proximodistal axis of the mandibular bone and surrounding lamina propria.
TS26: Meckel's cartilage	Present	Ubiquitous	3D	Staining is observed in along the entire axis.
TS26: tongue	Present	Widespread	3D	Staining is observed throughout the tongue, with strongest staining is the lamina propria.

Structure	Level	Pattern	Image	Note
TS26: mandible	Present	Widespread	3G	Staining is observed along the entire proximodistal axis of the mandible and surrounding lamina propria. Chondrocytes and surrounding peri- chondrium composing the anterior and lateral processes of the proximal mandible are also stained.
TS26: Meckel's cartilage	Present		3G	Staining is observed along the entire axis of Meckel's cartilage.

Structure	Level	Pattern	Image	Note
TS26: mandible	Present	Widespread	3G'	Staining is decreased near the junction of the processes (composed of chondrocytes and the perichondrium) with the mandibular bone. This is a zone of hypertrophic and ossified chondrocytes.

Structure	Level	Pattern	Image	Note
TS21: external auditory meatus	Present	Regionally restricted	4A	Staining is observed in the mesenchyme, but not in the epithelium.
TS21: Meckel's cartilage	Present		4A
TS21: middle ear	Present	Regionally restricted	4A
TS21: malleus pre-cartilage condensation	Present	Regionally restricted	4A	Staining is observed in cells in the distal malleus, but is absent in the cartilainous precursor of the malleus.
TS21: stapes pre-cartilage condensation	Present	Spotted	4A	Staining is observed in a mixture of cells of the caudal stapes and surrounding perichondrim, while most of the stapes is unlabeled.
TS21: sympathetic ganglion	Present	Scattered	4A'	Staining is observed in scattered cells.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present	Regionally restricted		Staining is observed in the cervical dorsal root ganglia.
TS21: peripheral nerve trunk	Present	Regionally restricted		Staining is observed in the superior cervical nerve trunk and the ventral primary ramus of the cervical nerve.

Structure	Level	Pattern	Image	Note
TS26: temporal bone	Present	Regionally restricted	4D	Staining is observed in almost all cells of the tympanic bone.
TS26: middle ear	Present	Regionally restricted	4D	Staining is observed in almost all cells of the gonial bone.
TS26: Meckel's cartilage	Present	Widespread	4D	Staining is observed in almost all cells of Meckel's cartilage.

Structure	Level	Pattern	Image	Note
TS26: malleus	Present	Regionally restricted	4G	Staining is confined to the manubrium of the malleus.
TS26: middle ear mesenchyme	Present		4G	Staining is observed in the mesenchyme surrounding the malleus.

Structure	Level	Pattern	Image	Note
TS26: incus	Absent		4J	Staining is not observed in the cartilaginous precursors of the stapes or incus.
TS26: stapes	Absent		4J	Staining is not observed in the cartilaginous precursors of the stapes or incus.

Structure	Level	Pattern	Image	Note
TS26: ear	Present	Regionally restricted	4M	Staining is observed in the distal portion of the styloid cartilage and cartilage associated with the pinna.
TS26: sympathetic ganglion	Present	Scattered	4M'	Staining is observed in scattered cells.

Structure	Level	Pattern	Image	Note
TS26: hyoid	Present	Regionally restricted	4P	Staining is observed, with the lesser horns of the hyoid composed exclusively of labeled cells, and the body composed of a mixture of labeled and unlabeled cells.
TS26: sublingual gland mesenchyme	Present		4P
TS26: submandibular gland mesenchyme	Present		4P
TS26: thyroid cartilage	Present	Scattered	4P	Staining is observed in scattered cells in the thyroid cartilage.
TS26: trunk	Present	Regionally restricted	4P , 4P'	Staining is observed in cells of the adipose tissue of the neck.

Structure	Level	Pattern	Image	Note
TS21: lower jaw	Present	Widespread	5A	Staining is observed in cells throughout the jaw.
TS21: Meckel's cartilage	Present	Widespread	5A
TS21: lower jaw incisor mesenchyme	Present		5A	Staining is observed in the proliferative area of the mesenchyme surrounding the incisor.
TS21: lower jaw incisor	Present	Regionally restricted	5A	Staining is not observed in the enamel organ of the incisor.
TS21: oral epithelium	Absent		5A

Structure	Level	Pattern	Image	Note
TS21: lower jaw	Present	Widespread	5D
TS21: lower jaw molar	Present	Regionally restricted	5D	Staining is not observed in the molar buds or the enamel organ.
TS21: lower jaw molar mesenchyme	Present		5D	Staining is observed in the proliferative areas around the molar.
TS21: Meckel's cartilage	Present		5D
TS21: oral epithelium	Absent		5D

Structure	Level	Pattern	Image	Note
TS23: lower jaw incisor epithelium	Present	Regionally restricted	5G	Staining is observed in part of the epithelium but is not present in the epithelial cap.
TS23: Meckel's cartilage	Present		5G	Staining is observed in the symphysis of Meckel's cartilage.
TS23: lower jaw incisor enamel organ	Absent		5G
TS23: lower jaw incisor dental papilla	Present		5G
TS23: lower jaw	Present		5G	Staining is observed in the mandibular bone and surrounding mesenchyme.
TS23: lower jaw mesenchyme	Present		5G	Staining is observed in the mandibular bone and surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: lower jaw	Present		5J
TS23: lower jaw mesenchyme	Present		5J
TS23: lower jaw incisor dental papilla	Present	Non-Uniform	5J
TS23: lower jaw incisor enamel organ	Absent		5J
TS23: lower jaw incisor dental lamina	Absent		5J

Structure	Level	Pattern	Image	Note
TS26: lower jaw incisor dental papilla	Present		5M
TS26: lower jaw	Present		5M	Staining is observed in the mandibular bone, Meckel's cartilage and surrounding mesenchyme.
TS26: lower jaw mesenchyme	Present		5M	Staining is observed in the mandibular bone, Meckel's cartilage and surrounding mesenchyme.
TS26: Meckel's cartilage	Present		5M
TS26: lower jaw tooth	Present	Regionally restricted	5M	Staining is absent from the developed enamel matrix.
TS26: oral epithelium	Absent		5M

Structure	Level	Pattern	Image	Note
TS26: lower jaw	Present		5P	Staining is observed in the mandibular bone.
TS26: lower jaw incisor dental papilla	Present		5P
TS26: lower jaw molar dental papilla	Present		5P
TS26: lower jaw incisor dental lamina	Absent		5P
TS26: lower jaw incisor enamel organ	Absent		5P
TS26: lower jaw molar dental lamina	Absent		5P
TS26: lower jaw molar enamel organ	Absent		5P
TS26: oral epithelium	Absent		5P
TS26: trigeminal nerve mandibular division	Absent		5P	Staining is not observed in the mandibular nerves.