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Gene Expression Data
Assay Details
Assay
Reference: J:79853 Liu C, et al., Pitx2c patterns anterior myocardium and aortic arch vessels and is required for local cell movement into atrioventricular cushions. Development. 2002 Nov;129(21):5081-91
Assay type: Recombinase reporter
MGI Accession ID: MGI:4834531
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Reporter: lacZ
Direct Detection
Results
Specimen Fig. 6A: embryonic day 10.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: cardiac muscle tissue Present 6A Staining is observed in cells in the outflow tract myocardium.
TS17: outflow tract Present 6A Staining is observed in cells in the outflow tract myocardium.
Specimen Fig. 6B: embryonic day 10.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2tm3.1Jfm (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: cardiac muscle tissue Present 6B Staining is observed in cells in the outflow tract myocardium.
TS17: outflow tract Present 6B Staining is observed in cells in the outflow tract myocardium.
Specimen Fig. 6C: embryonic day 12.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: cardiac muscle tissue Present Regionally restricted 6C Staining is observed in the myocardium overlying the interventricular groove.
TS20: interventricular groove Present Regionally restricted 6C Staining is observed in the myocardium overlying the interventricular groove.
TS20: outflow tract Present Regionally restricted 6C Staining is observed in some cells in the proximal outflow tract.
Specimen Fig. 6E: embryonic day 14.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: cardiac muscle tissue Present Regionally restricted 6E Staining is observed over more of the medial aspect of the heart.
TS22: heart Present Regionally restricted 6E Staining is observed over more of the medial aspect of the heart.
TS22: right ventricle cardiac muscle Present 6E Staining is observed in descesdents of right ventriucular and inner curvature myocardium.
Specimen Fig. 6G: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present Regionally restricted 6G Staining is observed over more of the medial aspect of the heart.
TS24: heart Present Regionally restricted 6G Staining is observed over more of the medial aspect of the heart.
TS24: right ventricle cardiac muscle Present 6G Staining is observed in descesdents of right ventriucular and inner curvature myocardium.
Specimen Fig. 6I: embryonic day 16.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant. Coronal section through heart.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present 6I Staining is observed in cells in the proximal outflow tract myocardium.
TS24: heart Present 6I Staining is observed in cells in the proximal outflow tract myocardium.
TS24: interatrial septum Present 6I Staining is observed in cells of the remodeled membranous and muscular septa.
TS24: interventricular septum Present 6I Staining is observed in cells of the remodeled membranous and muscular septa.
Specimen Fig. 6J: embryonic day 16.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant. Coronal section through heart.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present 6J Staining is observed in cells in the proximal outflow tract myocardium.
TS24: heart Present 6J Staining is observed in cells in the proximal outflow tract myocardium.
TS24: interatrial septum Present 6J Staining is observed in cells of the remodeled membranous and muscular septa.
TS24: interventricular septum Present 6J Staining is observed in cells of the remodeled membranous and muscular septa.
Specimen Fig. 6K: embryonic day 14.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: cardiac muscle tissue Present 6K Staining is observed in the right inner curvature myocardium.
TS22: heart Present 6K Staining is observed in the right inner curvature myocardium.
TS22: right ventricle cardiac muscle Present 6K
Specimen Fig. 6L: embryonic day 14.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2tm3.1Jfm (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: cardiac muscle tissue Present Regionally restricted 6L Staining is observed in fewer cells in the right inner curvature myocardium than in Pitx2<sup>+/-</sup> R26 mice.
TS22: heart Present Regionally restricted 6L Staining is observed in fewer cells in the right inner curvature myocardium than in Pitx2<sup>+/-</sup> R26 mice.
TS22: right ventricle cardiac muscle Present 6L Staining is observed in fewer cells in the right ventricular myocardium than in Pitx2<sup>+/-</sup> R26 mice.
Specimen Fig. 6M: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present Regionally restricted 6M Staining is observed in the right inner curvature myocardium.
TS24: heart Present Regionally restricted 6M Staining is observed in the right inner curvature myocardium.
TS24: right ventricle cardiac muscle Present 6M
Specimen Fig. 6N: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2tm3.1Jfm (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present Regionally restricted 6N Staining is observed in fewer cells in the right inner curvature myocardium than in Pitx2<sup>+/-</sup> R26 mice.
TS24: heart Present Regionally restricted 6N Staining is observed in fewer cells in the right inner curvature myocardium than in Pitx2<sup>+/-</sup> R26 mice.
TS24: right ventricle cardiac muscle Present 6N Staining is observed in fewer cells in the right ventricular myocardium than in Pitx2<sup>+/-</sup> R26 mice.
Specimen Fig. 6O: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant. Transverse section. Figure legend indicates age of E16.5 but text indicates E18.5.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present 6O Staining is observed in the myocardium.
TS24: heart Present 6O Staining is observed in the myocardium.
Specimen Fig. 6P: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2tm3.1Jfm (more )
Note: Conditional mutant. Transverse section. Figure legend indicates age of E16.5 but text indicates E18.5.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present Regionally restricted 6P No staining is observed in the myocardium near the cardiac apex.
TS24: heart Present Regionally restricted 6P No staining is observed in the myocardium near the cardiac apex.
Specimen Fig. 6Q: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant. Transverse section. Figure legend indicates age of E16.5 but text indicates E18.5.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present Regionally restricted 6Q Staining is observed in some cells in the myocardium of more caudal regions near the cardiac apex.
TS24: heart Present Regionally restricted 6Q Staining is observed in some cells in the myocardium of more caudal regions near the cardiac apex.
Specimen Fig. 6R: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2tm3.1Jfm (more )
Note: Conditional mutant. Transverse section. Figure legend indicates age of E16.5 but text indicates E18.5.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present Regionally restricted 6R No staining is observed in the myocardium near the cardiac apex.
TS24: heart Present Regionally restricted 6R No staining is observed in the myocardium near the cardiac apex.
Specimen Fig. 6S: embryonic day 12.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant. Transverse section.
Structure Level Pattern Image Note
TS20: atrioventricular endocardial cushion Present 6S Staining is observed in the AV cushion mesenchyme.
Specimen Fig. 6T: embryonic day 12.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2tm3.1Jfm (more )
Note: Conditional mutant. Transverse section.
Structure Level Pattern Image Note
TS20: atrioventricular endocardial cushion Absent 6T
Specimen Fig. 6U: embryonic day 14.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2tm3.1Jfm (more )
Note: Conditional mutant. Coronal section.
Structure Level Pattern Image Note
TS22: atrioventricular endocardial cushion Absent 6U
Specimen Fig. 6V: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2+ (more )
Note: Conditional mutant. Coronal section.
Structure Level Pattern Image Note
TS24: mitral valve leaflet Present 6V Staining is observed in cells of the AV valve leaflets.
TS24: tricuspid valve leaflet Present 6V Staining is observed in cells of the AV valve leaflets.
Specimen Fig. 6W: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Pitx2tm4(cre)Jfm/Pitx2tm3.1Jfm (more )
Note: Conditional mutant. Coronal section.
Structure Level Pattern Image Note
TS24: mitral valve leaflet Absent 6W No staining is observed in the AV valve leaflets.
TS24: tricuspid valve leaflet Absent 6W No staining is observed in the AV valve leaflets.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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