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Gene Expression Data
Assay Details
Assay
Reference: J:153498 Diez-Roux G, et al., A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol. 2011;9(1):e1000582
Assay type: RNA in situ
MGI Accession ID: MGI:4825621
Gene symbol: Irs4
Gene name: insulin receptor substrate 4
Probe: T36522
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramide-biotin/streptavidin amplification step was included in the in situ hybridization procedure.
Results
Specimen euxassay_011002_05: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: axial musculature Weak Regionally restricted euxassay_011002_05
Specimen euxassay_011002_06: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: axial musculature Weak Regionally restricted euxassay_011002_06
Specimen euxassay_011002_07: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: midbrain mantle layer Moderate Spotted euxassay_011002_07
Specimen euxassay_011002_08: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: midbrain mantle layer Moderate Spotted euxassay_011002_08
Specimen euxassay_011002_09: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: midbrain mantle layer Weak Spotted euxassay_011002_09
TS23: hypothalamus mantle layer Weak Spotted euxassay_011002_09
TS23: medulla oblongata basal plate mantle layer Weak Regionally restricted euxassay_011002_09
Specimen euxassay_011002_10: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: pituitary gland Strong Regionally restricted euxassay_011002_10
TS23: hypothalamus mantle layer Moderate Spotted euxassay_011002_10
TS23: midbrain mantle layer Moderate Spotted euxassay_011002_10
TS23: medulla oblongata basal plate mantle layer Weak Regionally restricted euxassay_011002_10
Specimen euxassay_011002_11: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: pituitary gland Strong Regionally restricted euxassay_011002_11
TS23: thyroid gland Strong Regionally restricted euxassay_011002_11
TS23: hypothalamus mantle layer Moderate Spotted euxassay_011002_11
TS23: medulla oblongata basal plate mantle layer Weak Regionally restricted euxassay_011002_11
Specimen euxassay_011002_12: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: pituitary gland Strong Regionally restricted euxassay_011002_12
TS23: medulla oblongata basal plate mantle layer Weak Regionally restricted euxassay_011002_12
Specimen euxassay_011002_13: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: pituitary gland Strong Regionally restricted euxassay_011002_13
Specimen euxassay_011002_14: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: pituitary gland Strong Regionally restricted euxassay_011002_14
TS23: thyroid gland Strong Regionally restricted euxassay_011002_14
TS23: hypothalamus mantle layer Moderate Spotted euxassay_011002_14
TS23: midbrain mantle layer Moderate Spotted euxassay_011002_14
TS23: medulla oblongata basal plate mantle layer Weak Regionally restricted euxassay_011002_14
Specimen euxassay_011002_15: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: pituitary gland Strong Regionally restricted euxassay_011002_15
TS23: thyroid gland Strong Regionally restricted euxassay_011002_15
TS23: midbrain mantle layer Moderate Spotted euxassay_011002_15
TS23: hypothalamus mantle layer Moderate Spotted euxassay_011002_15
TS23: medulla oblongata basal plate mantle layer Weak Regionally restricted euxassay_011002_15
Specimen euxassay_011002_16: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: pituitary gland Strong Regionally restricted euxassay_011002_16
TS23: midbrain mantle layer Moderate Spotted euxassay_011002_16
Specimen euxassay_011002_17: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: midbrain mantle layer Moderate Spotted euxassay_011002_17
Specimen euxassay_011002_19: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: axial musculature Weak Regionally restricted euxassay_011002_19
Specimen euxassay_011002_20: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: axial musculature Weak Regionally restricted euxassay_011002_20
Specimen euxassay_011002_21: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: axial musculature Weak Regionally restricted euxassay_011002_21
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory